Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration  

作　　者：Si-Min Xia Hui-Ming Yuan Zheng Liang Li-Hua Zhang Yu-Kui Zhang 

机构地区：[1]University of Chinese Academy of Science [2]National Chromatographic R&A Center,Dalian Institute of Chemical Physics,The Chinese Academy of Sciences

出　　处：《Chinese Chemical Letters》2015年第9期1068-1072,共5页中国化学快报（英文版）

基　　金：the financial support from National Basic Research Program of China(No.2012CB910604);National Natural Science Foundation of China(No.20935004);the Creative Research Group Project by NSFC(No.21321064);National High Technology Research and Development Program of China (No.2012AA020202)

摘　　要：In this work,a novel kind of particulate capillary precolumns with double-end polymer monolithic frits has been developed.Firstly,the polymer monolithic frit at one end was prepared via photo-initiated polymerization of a mixture of lauryl methacrylate and ethyleneglycol dimethacrylate with 1-propanol and 1,4-butanediol as porogens and 2,2-dimethoxy-2-phenylacetophenone as a photo-initiator in UV transparent coating capillary（100 μm i.d.）.Subsequently,C18 particles（5 μm,100 A） were packed into the capillary,and sealed with the polymer monolithic frit at another end.To prevent the reaction of monomers and C18 particles,the packed C18 particles were masked during UV exposure.The loading capacity of such a precolumn was determined to be about 9 μg by frontal analysis with a synthetic peptide APGDR1 YVHPF as a model sample.Furthermore,two parallel precolumns were incorporated into a two-dimensional nano-liquid chromatography（2D nano-LC） system with dual capillary trap columns for peptide trapping and concentration.Compared to 2D nano-LC system with a single trap column,such two dimensional separations could be operated simultaneously to improve the analysis throughput.All these results demonstrated that such capillary precolumns with double frits would be promising for high-throughput proteome analysis.In this work,a novel kind of particulate capillary precolumns with double-end polymer monolithic frits has been developed.Firstly,the polymer monolithic frit at one end was prepared via photo-initiated polymerization of a mixture of lauryl methacrylate and ethyleneglycol dimethacrylate with 1-propanol and 1,4-butanediol as porogens and 2,2-dimethoxy-2-phenylacetophenone as a photo-initiator in UV transparent coating capillary（100 μm i.d.）.Subsequently,C18 particles（5 μm,100 A） were packed into the capillary,and sealed with the polymer monolithic frit at another end.To prevent the reaction of monomers and C18 particles,the packed C18 particles were masked during UV exposure.The loading capacity of such a precolumn was determined to be about 9 μg by frontal analysis with a synthetic peptide APGDR1 YVHPF as a model sample.Furthermore,two parallel precolumns were incorporated into a two-dimensional nano-liquid chromatography（2D nano-LC） system with dual capillary trap columns for peptide trapping and concentration.Compared to 2D nano-LC system with a single trap column,such two dimensional separations could be operated simultaneously to improve the analysis throughput.All these results demonstrated that such capillary precolumns with double frits would be promising for high-throughput proteome analysis.

关 键 词：Capillary precolumn Double-end frits Peptide trapping 2D nano-LC 

分 类 号：Q51[生物学—生物化学]