Lineal DNA logic gate for microRNA diagnostics with strand displacement and fluorescence resonance energy transfer  

作　　者：Tian-Tian Meng Ying-Xin Liu Meng-Tan Liu Jiao-Bao Long Qing-Feng Cao Shu-Ya Yan Xiang-Xian Meng 

机构地区：[1]College of Biology,The High School Attach to Hunan University [2]Hunan University [3]School of Life Science,Xiamen University

出　　处：《Chinese Chemical Letters》2015年第9期1179-1182,共4页中国化学快报（英文版）

基　　金：supported by National Natural Science Foundation of China(No.21275043);National Basic Research Program of China under Grants(No.2009CB421601)

摘　　要：Designing molecular logic gates to operate programmably for molecular diagnostics in molecular computing still remains challenging.Here,we designed a novel linear DNA logic gates for microRNA analysis based on strand displacement and fluorescence resonance energy transfer（FRET）.Two labeled strands closed each other produce to FRET through hybridization with a complementary strand to form a basic work unit of logic gate.Two indicators of heart failure（microRNA-195 and microRNA-21） were selected as the logic inputs and the fluorescence mode was used as the logic output.We have demonstrated that the molecular logic gate mechanism worked well with the construction of YES and AND gates.Designing molecular logic gates to operate programmably for molecular diagnostics in molecular computing still remains challenging.Here,we designed a novel linear DNA logic gates for microRNA analysis based on strand displacement and fluorescence resonance energy transfer（FRET）.Two labeled strands closed each other produce to FRET through hybridization with a complementary strand to form a basic work unit of logic gate.Two indicators of heart failure（microRNA-195 and microRNA-21） were selected as the logic inputs and the fluorescence mode was used as the logic output.We have demonstrated that the molecular logic gate mechanism worked well with the construction of YES and AND gates.

关 键 词：Molecular logic gate Strand displacement Fluorescence resonance energy transfer 

分 类 号：R440[医药卫生—诊断学]