HPLC法测定有瓜石斛中原儿茶酸的含量  

Determination of content of protocatechuic acid in Herba flickingeriae by HPLC

作　　者：朱莎[1,2] 任晋[2] 谢镇山 黄月纯[3] 魏刚[2] 

机构地区：[1]广州医科大学第一附属医院,广州510120 [2]广州中医药大学,广州510006 [3]广州中医药大学第一附属医院,广州510405

出　　处：《北方药学》2015年第10期1-2,共2页Journal of North Pharmacy

摘　　要：目的：建立有瓜石斛中原儿茶酸的含量测定方法。方法：采用高效液相色谱（HPLC）法。色谱柱为Zorbax SB-Aq色谱柱;流动相为乙腈-0.1%磷酸溶液;检测波长为220nm;流速为1.0m L·min-1;柱温为30℃。结果：原儿茶酸达到基线分离,线性关系良好（r=1.0000）;平均加样回收率为101.71%,RSD为2.48%（n=6）。12批市售样品含量在0.0374～0.203mg·g^-1范围内,差异相对较大。结论：该方法灵敏,可靠,可用作有瓜石斛药材的质量评价指标之一。Objective：To establish a method for determintation of protocatecbuic acid in Herba flickingeriae by HPLC. Methods：HPLC method was used with the Zorbax SB-Aq column, the acetonitrile-0.1% phosphoric acid was employed as a mobile phase, detection wavelength was 220nm. Column temperature was 30℃, and flow rate was 1.0mL·min^-1. Results：Baseline separation was obtained and linear relationship was good （r=-1.0000） for protocatechuic acid content. The average recovery was 101.71% and RSD was 2.48%（n=6）. The content of protocatechuic acid in the 12 samples of commercially available Herba flickingeriae were among 0.0374～0.203mg·g^-1, with distinct difference. Conclusion ：The method is sensitive, reliable and available for the quality control of Herba flickingeriae.

关 键 词：有瓜石斛 原儿茶酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]