花鲈摄食调控相关因子全长cDNA的克隆和序列分析  

Cloning and Sequence Analysis of Full-Length cDNAs of Food Intake Regulation Factors in Japanese Seabass(Lateolabrax japonicus)

作　　者：刘艳[1,2] 许恒龙[1] 薛敏[2,3] 王嘉[2] 吴秀峰[2] 郑银桦[2] 韩芳[2] 张彦娇[4] 

机构地区：[1]中国海洋大学,微型生物生态学研究室,青岛266003 [2]中国农业科学院饲料研究所,国家水产饲料安全评价基地,北京100081 [3]农业部饲料生物技术重点开放实验室,北京100081 [4]中国海洋大学农业部水产动物营养与饲料重点实验室和海水养殖教育部重点实验室,青岛266003

出　　处：《动物营养学报》2015年第9期2757-2773,共17页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31372539);公益性行业(农业)专项经费项目(201203015);北京市现代农业产业技术体系(SCGWZJ20151103-1);国家重点基础研究发展计划(973计划)(2014CB138601)

摘　　要：本试验采用反转录聚合酶链式反应（RT-PCR）和cDNA末端快速扩增（RACE）技术克隆花鲈（Lateolabrax japonicus）调控摄食因子雷帕霉素靶蛋白（mTOR）、核糖体蛋白S6激酶beta-1（S6K1）、神经肽Y前体（NPY precursor）和脑肠肽前体（preproghrelin）cDNA全长序列。结果显示：花鲈mTOR（GenBank登录号KJ746670）cDNA全长8 296bp,开放阅读框7 551bp,编码2 516个氨基酸,预测其分子质量为286.14ku,与其他物种的相似性为62.0%～98.0%;S6 K1（GenBank登录号KJ746671）cDNA全长2 232bp,开放阅读框1 539bp,编码512个氨基酸,预测其分子质量为56.87ku,与其他物种的相似性为80.4%～84.9%;NPY precursor（GenBank登录号KJ850326）cDNA全长676bp,开放阅读框300bp,编码99个氨基酸,预测其分子质量为11.26ku,与其他物种的相似性为53.6%～99.0%;preproghrelin（GenBank登录号KJ850327）cDNA全长1 201bp,开放阅读框324bp,编码107个氨基酸,预测其分子质量为12.03ku,与其他物种的相似性为19.6%～85.0%。花鲈摄食调控因子cDNA全长序列地获得将为进一步研究其摄食调控机理奠定基础。The full-length cDNAs of food intake regulation factors,including mechanistic target of rapamycin（mTOR）,ribosomal protein S6 kinase beta-1（S6 K1）,neuropeptide Y（NPY）precursor and preproghrelin were cloned from Japanese seabass（Lateolabrax japonicus）using reverse transcription-polymerase chain reaction（RT-PCR）and rapid amplification of cDNA end（RACE）methods.The results showed as follows：the full-length mTOR cDNA of Japanese seabass（GenBank accession No.KJ746670）was 8 296 bp,consisting of an open reading frame（ORF）of 7 551 bp encoding apolypeptide of 2 516 amino acids with a theoretical molecular weight of 286.14 ku,and shared 62.0%to 98.0%similarity with other species.The full-length S6 K1 cDNA of Japanese seabass（GenBank accession No.KJ746671）was2 232 bp,consisting of an ORF of 1 539 bp encoding apolypeptide of 512 amino acids with a theoretical molecular weight of 56.87 ku,and shared 80.4%to 84.9%similarity with other species.The full-length NPY precursor cDNA of Japanese seabass（GenBank accession No.KJ850326）was 676 bp,consisting of an ORF of 300 bp encoding apolypeptide of 99 amino acids with a theoretical molecular weight of 11.26 ku,and shared 53.6% to 99.0% similarity with other species.The full-length preproghrelin cDNA of Japanese seabass（GenBank accession No.KJ850327）was 1 201 bp,consisting of an ORF of 324 bp encoding apolypeptide of 107 amino acids with a theoretical molecular weight of 12.03 ku,and shared 19.6%to 85.0% similarity with other species.The results of this study will provide a scientific basis for further study to explain regulatory mechanism of the factors of food intake regulation in Japanese seabass.

关 键 词：基因克隆 摄食调控 花鲈 雷帕霉素靶蛋白 核糖体蛋白S6激酶beta-1 神经肽Y前体 脑肠肽前体 

分 类 号：S963[农业科学—水产养殖]