不明原因复发性自然流产患者FOXP3基因蛋白表达与启动子甲基化水平之间关系的研究  

Association between FOXP3 promoter methylation and the expression of FOXP3 protein in unexplained recurrent spontaneous abortion

作　　者：侯文汇 李银广[1] 李珠玉[1] 李婕[1] 方利元[2] 李小青[1] 游泽山[1] 

机构地区：[1]中山大学附属第一医院妇产科,广州510080 [2]广东省妇幼保健院,广州510010

出　　处：《新医学》2015年第9期580-583,共4页Journal of New Medicine

基　　金：广东省自然科学基金(S2011010003522)

摘　　要：目的探讨不明原因复发性自然流产(URSA)患者蜕膜组织中叉头样转录因子P3(FOXP3)基因的蛋白表达水平及其与启动子甲基化水平的关系。方法利用蛋白印迹法检测20例URSA患者(URSA组)和20名正常妊娠主动要求人工流产术的健康妇女(正常对照组)蜕膜组织中FOXP3蛋白表达情况,利用结合重亚硫酸盐的测序法(BSP)检测2组FOXP3基因启动子甲基化情况,并分析两者的相关性。结果 URSA组蜕膜组织中FOXP3表达水平明显低于正常对照组(P<0.01)。BSP显示URSA组FOXP3基因启动子甲基化水平明显高于正常对照组(P<0.01)。FOXP3蛋白表达水平与FOXP3基因启动子甲基化水平呈负相关(r=-0.917,P<0.01)。结论 FOXP3基因启动子甲基化水平升高后,FOXP3基因蛋白表达水平下调,引起免疫耐受异常,可能导致RSA的发病。Objective To investigate the association between the level of FOXP3 promoter methyla-tion and the expression level of FOXP3 protein in the decidua of patients with unexplained recurrent spontane-ous abortion （URSA）.Methods The expression levels of FOXP3 protein in the decidua of 20 URSA patients and 20 healthy counterparts who underwent induced abortion were assessed by Western blot.Bisulfite-assisted genomic sequencing PCR （BSP）was utilized to detect the levels of FOXP3 promoter methylation.The correla-tion between two indexes was evaluated.Results The expression level of FOXP3 in the decidua of the URSA group was significantly lower than that of the control group （P 〈0.01）,whereas the level of FOXP3 promoter methylation in the URSA group was significantly higher compared with that in the control group （P 〈0.01）.The expression level of FOXP3 protein was negatively correlated with the level of FOXP3 promoter methylation （r =-0.917,P 〈0.01）.Conclusion As the level of FOXP3 promoter methylation increases,the expres-sion level of FOXP3 protein is down-regulated,which may cause abnormal immune tolerance and potentially lead to the incidence of RSA.

关 键 词：不明原因复发性自然流产 叉头样转录因子 P3 基因启动子 甲基化 FOXP3 

分 类 号：R714.21[医药卫生—妇产科学]