丙型肝炎病毒NS3-5B转基因小鼠模型的构建  

Generation of the Transgenic Mice Model Expressing HCV NS3-5B

作　　者：赵海卫[1] 韩佩君[1] 姚敏[1] 吕欣[1] 雷迎峰[1] 杨敬[1] 乔卿华[1] 翁代慧[1] 党品香 尹文[2] 

机构地区：[1]第四军医大学基础医学部微生物学教研室,陕西西安710032 [2]第四军医大学西京医院输血科,陕西西安710032

出　　处：《生物技术通讯》2015年第5期627-631,共5页Letters in Biotechnology

基　　金：陕西省科技统筹创新工程计划(2014KTCL03-08);军队后勤计划(CWS13L058)

摘　　要：目的:构建表达丙型肝炎病毒(HCV)NS3-5B蛋白的转基因小鼠模型。方法:显微注射线性化p IRES2-EGFP-NS3-5B载体到小鼠受精卵,制备并传代筛选HCV NS3-5B转基因小鼠;通过血清谷丙转氨酶(ALT)、谷草转氨酶(AST)检测和肝脏HE染色,对6周龄转基因小鼠进行肝功能评价。结果:PCR、RT-PCR和Western印迹结果表明HCV NS3-5B转基因小鼠构建成功;6周龄部分转基因小鼠血清ALT和AST值升高,但差别无统计学意义,肝组织形态无改变。结论:构建了HCV NS3-5B转基因小鼠模型,为进一步在体研究HCV复制复合体建立了平台。Objective： To construct the transgenie mice model expressing HCV NS3-5B protein. Methods： HCV NS3-5B transgenic mice were generated by microinjection of the linearized vector of pIRES2-EGFP-NS3-5B into mice fertilized eggs. Subsequently, homozygous transgenic mice with genetic stability were selected by breeding. Liv- er function of 6 weeks old transgenic mice were evaluated via testing ALT and AST in serum and HE staining of liver. Results： NS3-5B transgenic mice were identified by PCR, RT-PCR and Western blotting. Compared with the same age of wild type C57BL/6, the change of ALT and AST of the transgenic mice were no statistical differ- ence and the shape of liver tissue was unchanged. Conclusion： The transgenic mouse model expressing HCV NS3- 5B was generated successfully. It provides a research platform for HCV replication complex in vivo.

关 键 词：丙型肝炎病毒 NS3-5B蛋白 复制复合体 转基因小鼠模型 

分 类 号：Q78[生物学—分子生物学]