雪莲培养物高效液相色谱指纹图谱研究  

Fingerprint of Tissue Culture of Saussurea medusa Maxim by HPLC

作　　者：贾康杰 马昆鹏[2] 高丽华[3] 邵勇[3] 方均建[2] 董芳霆[2] 胡显文[3] 

机构地区：[1]山西康宝生物制品股份有限公司,山西长治046000 [2]国家生物医学分析中心,北京100850 [3]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2015年第5期682-686,共5页Letters in Biotechnology

摘　　要：目的:采用高效液相色谱法(HPLC)建立雪莲培养物指纹图谱。方法:以紫丁香苷为参照物,采用梯度洗脱HPLC法建立雪莲培养物指纹图谱,并进行相似度评价和聚类分析。色谱柱为CAPCELL PAK C18(4.6 mm×250mm,5μm),流动相A为0.1%TFA/水,流动相B为0.1%TFA/乙腈,梯度洗脱,检测波长260 nm。结果:建立了雪莲培养物指纹图谱,方法的精密度、稳定性、重现性较好,共标示出9个共有峰。结论:该法为雪莲培养物的鉴定提供了依据,其指纹图谱可用于雪莲培养物的质量控制。Objective： To establish a method of HPLC fingerprint analysis of tissue culture of Saussurea medusa Maxim. Methods： With syringin as the reference substance, gradient elution HPLC method was applied to estab- lish fingerprint chromatography of tissue culture of S.medusa Maxim, and the similarity evaluation and cluster analy- sis. CAPCELL PAK C18（4.6 mm×250mm, 5 μm） was used. Mobile phase A（water-0.1% TFA） and mobile phase B （acetonitrile-0.1% TFA） were adopted as the mobile phase, with gradient elution. Detection wavelength was 260 nm. Results： The method had good precision, stability and repeadability. Nine common peaks were found in the HPLC fingerprint of tissue culture of S.medusa Maxim. Conclusion： The method provides evidence for the identification of tissue culture of S.medusa Maxim. The fingerprint can be used to control the quality of tissue cul- ture of S.medusa Maxim.

关 键 词：雪莲培养物 指纹图谱 紫丁香苷 高效液相色谱法 

分 类 号：Q502[生物学—生物化学]