microRNA-7对小鼠肠系膜淋巴结αβT淋巴细胞组成的影响  

Effect of microRNA-7 on αβT lymphocytes of mouse mesenteric lymph nodes

作　　者：赵娟娟[1] 朱顺飞 徐华林[1] 胡燕[1] 郭萌萌[1] 陶弋婧 周涯[2] 秦娜琳[1] 郑静[1] 徐林[1] 

机构地区：[1]遵义医学院免疫学教研室暨贵州省免疫学研究生教育创新基地,遵义563000 [2]遵义医学院医学物理学教研室,遵义563000

出　　处：《中国免疫学杂志》2015年第9期1163-1168,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.31370918);教育部"新世纪优秀人才计划"项目(NCET-12-0661)资助

摘　　要：目的:研究miR-7(MicroRNA-7,miRNA-7)对小鼠肠系膜淋巴结αβT淋巴细胞比例的影响并初步探讨其意义。方法:利用miR-7基因敲减(Knockdown,KD)小鼠模型,观察其肠系膜淋巴结大小、重量和淋巴结细胞总数,以及HE染色的病理学变化;流式细胞术(FACS)检测淋巴结中αβT淋巴细胞亚群(CD3+T细胞、CD4+T细胞和CD8+T细胞)以及CD4+T细胞和CD8+T细胞的活化相关分子CD69、CD62L和功能相关因子IFN-γ表达的情况。结果:与野生型(Wild type,WT)小鼠相比,miR-7KD小鼠肠系膜淋巴结大小、重量指数和淋巴结细胞总数均显著增高(P<0.05),且HE染色显示MLNs有病理性改变;肠系膜淋巴结中αβCD3+T细胞比例增加显著,其中以CD4+T细胞比例变化最为明显(P<0.05),而CD19+B淋巴细胞比例明显下调(P<0.05),但各亚群细胞总数均明显增加(P<0.05);最后,CD4+T细胞和CD8+T细胞中的CD62L+细胞的比例均显著下调(P<0.05),而CD69+细胞和IFN-γ+细胞比例均显著上调(P<0.05)。结论:miR-7敲减后可显著影响肠系膜淋巴结中αβT淋巴细胞的组成比例,提示其对肠系膜淋巴结中淋巴细胞的组成和功能具有重要的调控作用。Objective：To detect the change of proportions of αβT lymphocytes in mesenteric lymph nodes of miR-7 knockdown （miR-7KD）mice,and preliminarily explore its importance.Methods： The volume,weight index and total cells number of mesenteric lymph node in miR-7KD mice were measured.The pathologic morphology change of mesenteric lymph nodes was observed by HE stai -ning.And the changes on proportion of αβT lymphocytes in mesenteric lymph nodes of miR-7KD mice were analyzed by Flow cytometry.Results：Compared with those of WT （wild type） mice,the volume,weight index,and the total cells number of mesenteric lymph node were significantly increased （ P〈0.05 ）.Moreover ,the pathologic morphology was significantly changed.The proportion and numbers of T lymphocyte were significantly increased;however,the proportion of B lymphocyte were significantly decreased （P〈0.05）. Notably,the proportion and number of CD4^＋T cells and CD8^＋T cells were significantly increased （P〈0.05）.Meanwhile,CD62L^＋T cell proportion were vigorously reduced and CD 69^＋T cell and IFN-γ^＋T cell proportion were notably up-regulated,which belong to CD4^＋and CD8^＋T cell（P〈0.05）.Conclusion：There was significant influence in the proportion of αβT lymphocytes in mesenteric lymph nodes of miR-7KD mice,suggesting that miR-7 might play an important role in the composition and function of lymphocytes in mesenteric lymph nodes.

关 键 词：miR-7基因敲减 肠系膜淋巴结 ΑΒT细胞 

分 类 号：R392[医药卫生—免疫学]