参附注射液对脑缺血再灌注损伤大鼠Nrf2信号通路的影响  

Effect of Shenfu injection on Nrf2 signaling pathway in rats with cerebral ischemia reperfusion-induced brain injury

作　　者：江承平[1] 吴碧华[2] 王柏强[3] 罗飞[3] 何效平[3] 王晓明[2] 刘黎明[2] 

机构地区：[1]川北医学院药学院,南充637003 [2]川北医学院神经病学研究所,南充637003 [3]川北医学院附属医院药剂科,南充637007

出　　处：《中国免疫学杂志》2015年第9期1191-1194,共4页Chinese Journal of Immunology

基　　金：四川省科技厅资助项目(2010JY0094)

摘　　要：目的:探讨参附注射液治疗在大鼠脑缺血再灌注损伤中的保护作用及其对Nrf2信号通路的影响。方法:将68只雄性SD大鼠随机分为假手术组、脑缺血再灌注组、缺血再灌注损伤加参附注射液(8 mg/kg)治疗组,采用线栓法制作大鼠大脑中动脉缺血再灌注损伤模型,再灌注后24 h对大鼠进行行为学评分后处死并取脑,采用免疫印迹法检测参附注射液对Nrf2、HO-1、NQO1和cleaved-caspase-3蛋白表达的影响,尼氏法检测脑缺血再灌注损伤后的神经元损伤程度。结果:参附注射液治疗可以显著增加核内Nrf2蛋白的表达(P<0.05),显著增加脑缺血再灌注损伤围脑组织中HO-1、NQO-1的表达量(P<0.05),明显减少cleaved-caspase-3蛋白的表达和受损神经元的个数,显著改善神经功能评分(P<0.05)。结论:参附注射液可以通过上调Nrf2信号通路从而在脑缺血再灌注损伤中发挥神经保护作用。Objective：To study the neuro-protective effects of Shenfu injection on Nrf 2 signaling pathway affected by cerebral ischemia reperfusion.Methods： A total of 68 adult male Sprague-Dawley rats were randomly divided into sham group , cerebral ischemia-reperfusion group,and（8 mg/kg）Shenfu injection treatment group.Shenfu injection was injected intraperitoneally in the rats after MCAO.Neurologic deficit was evaluated after 24 hours of reperfusion.All the rats were sacrificed after 24 h of ischemia-reoerfusion for biochemical analysis or Nissl staining.Results：Shenfu injection treatment significantly increased the expression of Nrf 2,HO-1 and NQO-1（P〈0.05）.Furthermore,Shenfu injection treatment significantly reduced the expression of cleaved -caspase-3 and attenuated neurological deficits after cerebral ischemia reperfusion （ P〈0.05 ）.Lastly, Shenfu injection could evidently alleviate the severity of neuronal degeneration （ P〈0.05 ）.Conclusion：Shenfu injection could confer neuroprotection after cerebral ischemia reperfusion through modulating the Nrf2 signaling pathway.

关 键 词：脑缺血再灌注损伤 参附注射液 NRF2 大鼠 

分 类 号：R285.5[医药卫生—中药学] R743.3[医药卫生—中医学]