结肠癌组织VEGF表达与树突状细胞浸润的临床意义及与血清VEGF浓度及其活化水平的相关性  

Clinical significance of VEGF expression in colon cancer tissue and dendritic cells in colon carcinoma and correlation analysis of serum VEGF and DCs activation

作　　者：张子杰[1] 

机构地区：[1]河南省南阳市中心医院病理科,南阳473009

出　　处：《中国免疫学杂志》2015年第9期1257-1261,共5页Chinese Journal of Immunology

摘　　要：目的:研究结肠癌组织VEGF表达与树突状细胞(DCs)浸润的临床意义,并且探讨其与外周血清VEGF水平及DCs活化水平的相关性。方法:采用免疫组化法测定结肠癌和癌旁组织内VEGF和S-100蛋白的表达。采用酶联免疫吸附实验测定结肠癌患者术前及健康受试者外周血VEGF水平,分离结肠癌患者术前和健康受试者外周血单核细胞,利用TNF-α体外诱导为DCs,流式细胞仪检测DCs表面CD83的表达,分析结肠癌组织VEGF表达与DCs浸润的临床意义以及与外周血清VEGF水平及DCs活化水平的相关性。结果:1结肠癌组织VEGF阳性表达率较结肠癌旁组织显著提高(P<0.01),S-100蛋白阳性表达率较结肠癌旁组织显著降低(P<0.01);2结肠癌患者外周血VEGF水平较健康受试者显著升高(P<0.01),而外周血DCs中CD83水平较健康受试者显著降低(P<0.01);3结肠癌组织VEGF和S-100蛋白的表达水平与肿瘤的分化、分期和淋巴结转移程度相关(P<0.01);4结肠癌组织VEGF表达与S-100表达呈负相关(P<0.01);5结肠癌组织VEGF表达与外周血VEGF正相关(P<0.01),与DCs活化水平负相关;6结肠癌组织DCs浸润程度与外周血VEGF负相关(P<0.01),与DCs活化水平正相关(P<0.01)。结论:结肠癌组织VEGF表达和DCs浸润共同参与结肠癌的进展,并且VEGF可抑制DCs浸润和活化。Objective：To investigate the clinic significance of vascular endothelial cell growth factor （ VEGF） expression and dendritic cells （ DCs ） infiltration in colon cancer tissues , and to analysis the correlation between the sera VEGF with DCs activation.Methods：The expressions of VEGF and S-100 protein in colon carcinoma tissue and pericarcinous tissue were determined by immumohistochemistry.The serum VEGF level of colon carcinoma patients in preoperation and healthy subjects was detected using ELISA.Peripheral blood monocytes were isolated in preoperative patients and healthy subjects and induced to DCs by TNF -αin vitro.The surface expression of CD 83 in DCs was assayed with flow cytometry.Results： Expression of VEGF protein positive in colon carcinoma tissue was significantly higher than the pericarcinous tissue （P〈0.01）,while S-100 positive expression of colon carcinoma tissue was significantly lower than the pericarcinous tissue （ P〈0.01 ）.The serum VEGF level in colon carcinoma patients was significantly higher than healthy subjects （ P〈0.01 ） ,while the CD83 level in peripheral blood DCs was significantly lower than healthy subjects （ P〈0.01 ）.The expression of VEGF and S-100 protein in colon carcinoma tissue was correlated with tumor differentiation , stage and lymph node metastasis （ P〈0.01 ）.The expression of VEGF was inversely correlated with S-100 （ P〈0.01 ） in colon carcinoma tissue , while positively correlated with the serum VEGF （ P〈0.01 ） and inversely correlated with DCs activation.The infiltration degree of DCs was inversely correlated with serum VEGF , and positively correlated with the DCs activation.Conclusion：VEGF expression in colon carcinoma tissue and DCs infiltration co-participate in the progress of colon cancer ,and VEGF could inhibit the infiltration and activation of DCs.

关 键 词：结肠癌 血管内皮细胞生长因子 树突状细胞 相关性 

分 类 号：R392.32[医药卫生—免疫学]