阿里红多糖联合复方新诺明治疗大鼠卡氏肺孢子菌肺炎的实验研究  

Experimental study of fomes officinalis polysaccharides and cotrimoxazole in treating pneumocystis carinii pneumonia in rats

作　　者：刘云飞[1] 赖星星[1] 孙艳[1] 陈永杰[1] 伊丽孜拉.伊克拉木 高剑[2] 

机构地区：[1]新疆医科大学 [2]新疆医科大学基础医学院人体寄生虫学教研室

出　　处：《医学动物防制》2015年第8期863-866,F0004,共5页Journal of Medical Pest Control

基　　金：国家级大学生创新创业训练计划项目(201310760002);新疆医科大学大学生创新性实验计划项目(CX2013002);新疆医科大学博士(后)科研项目启动基金并新疆医科大学科研创新基金(XJ201222)

摘　　要：目的探讨维药阿里红多糖(Fo P)单独或联合复方新诺明对大鼠卡氏肺孢子虫肺炎(Pneumocystis Carinii Pneumonia,PCP)的治疗作用。方法以连续8周皮下注射地塞米松的方法建立SD大鼠PCP动物模型,随机分为9组:PCP模型对照组,阿里红多糖(Fo P)低(14 mg/kg)、中(28 mg/kg)、高剂量(56 mg/kg)组,复方新诺明治疗对照组,各剂量Fo P与复方新诺明联合用药组,正常对照组。通过各组大鼠平均体重变化、平均肺重、肺重/体重比、肺脏组织学变化和肺脏印片包囊计数等指标评价疗效。结果阿里红多糖(Fo P)单独作用或与复方新诺明联合治疗均能显著恢复PCP大鼠体重;高剂量Fo P联合治疗组不但减轻PCP大鼠肺脏病理损伤效果最明显,而且肺脏印片中PC包囊减少率可达87.92%,印片中PC包囊数量为(7.67±5.24),而PCP模型组大鼠肺脏印片中PC包囊数量为(62.83±19.42),差异有统计学意义(F=19.450,P=0.00);复方新诺明对照组大鼠肺脏印片中PC包囊数量为(23.83±16.36),中、低剂量Fo P联合治疗组、高剂量Fo P治疗组与该组相比差异无统计学意义(P=0.122);中、低剂量Fo P单独治疗组无法减少PCP大鼠肺脏中PC包囊数量。结论维药阿里红多糖(Fo P)和复方新诺明联合用药治疗卡氏肺孢子菌肺炎大鼠较单用复方新诺明效果显著,Fo P本身没有直接抑制虫体增殖的作用,主要通过调节大鼠免疫功能发挥治疗PCP的作用。Objective To explore and research the therapeutic efficacy of fomes officinalis polysaccharides（ Fo P） and cotrimoxazole on rats with pneumocystis carinii pneumonia（ PCP）. Methods PCP animal models were established by hypodermic injection of dexamethasone in rats for 8 weeks,and then divided the model into nine groups randomly： PCP model control group,Low dose Fo P group（ 14 mg / kg）,Moderate dose Fo P group（ 28 mg / kg）,High dose Fo P group（ 56 mg / kg）,cotrimoxazole control group,and each dose Fo P combined treatment group. At the same time normal control group was established. The therapeutic effect of each treatment group was evaluated by rat weight,lung weight,lung / body weight ratio,pathological changes and pneumocystis carinii（ PC） cyst count in lung printing of rats. Results Both the single FOP groups and the cotrimoxazole combined groups could significantly restore body weight of PCP rats; High- dose Fo P combination therapy group had the most obvious effect on the repair of lung tissue damage, and cysts reduction rate reached to 87. 92%. The number of PC cysts of rats in high- dose Fo P combined treatment group was（ 7. 67± 5. 24）,and the number of PC cysts of rats in PCP model control group was（ 62. 83 ± 19. 42）. There were significant differences in the number of cysts between two groups（ F = 19. 450,P = 0. 00）. The number of PC cysts of rats in cotrimoxazole group was（ 23. 83 ± 16. 36）. No statistical significance found between the low- dose Fo P combined treatment group,the moderate- dose Fo P combined treatment group,high- dose Fo P group and the cotrimoxazole control group（ P = 0. 122）. The low- dose Fo P group and the moderate-dose Fo P group couldn＇t reduce the number of PC cysts in lung tissue of PCP rats. Conclusions The combined treatment of Fo P and cotrimoxazole had a more significant therapeutic efficacy on rat with pneumocystis carinii pneumonia than that with cotrimoxazole independently. Fop doesn＇t directly inhibit the proliferation o

关 键 词：阿里红多糖 卡氏肺孢子虫肺炎 复方新诺明 联合治疗 

分 类 号：R519.8[医药卫生—内科学]