先天性小耳畸形差异性长链非编码RNA表达谱与生物信息学分析  

LncRNAs expression profiles in microtia and bioinformatics analysis

作　　者：张玲[1] 林琳[1] 宋宇鹏[1] 毕晔[1] 潘博[1] 杨庆华[1] 蒋海越[1] 

机构地区：[1]北京协和医学院中国医学科学院整形外科医院整形七科,北京100144

出　　处：《中国优生与遗传杂志》2015年第10期23-26,共4页Chinese Journal of Birth Health & Heredity

基　　金：北京市自然基金(7132176);协和青年基金(33320140171);国家自然科学基金(81272124;31040031)

摘　　要：目的探讨分析先天性小耳畸形差异性长链非编码RNA(lnc RNA)的表达谱与生物信息学分析。方法采用Arraystar高通量lnc RNAs基因芯片研究3例先天性小耳畸形的患者的小耳侧残耳软骨与正常侧耳软骨,获得lnc RNAs差异性表达谱。结果本实验所用的基因芯片包含30 586个lnc RNAs和26 109个m RNAs探针;与正常侧软骨比较,残耳软骨共检测出差异性表达Lnc RNAs共有180条,其中表达上调的Lnc RNAs有74条,表达下调的Lnc RNAs有106条。信号通路分析显示涉及到甘油酯类代谢、破骨细胞分化、肿瘤生长等。结论先天性小耳畸形存在明显的lnc RNAs差异性表达,这些lnc RNAs及某些信号通路可能在小耳畸形发生和发展中发挥重要作用。Objective：To analyze lnc RNA expression profiles in microtia and bioinformatics analysis. Methods：Three pairs of the residual ear cartilage and normal ear cartilage of 3 patients with microtia were studied by using high-throughput lnc RNAs Arraystar gene chip,obtain lnc RNAs differential expression profiles. Results：Gene chip used in this study included 30 586 lnc RNAs and 26 109 m RNAs probe;Compared with normal cartilage,180 lnc RNAs differential expression were detected in the residual ear cartilage 74 up-regulated expression lnc RNAs and 106 down-regulated expression. Signal path analysis involves glyceride metabolism,osteoclast differentiation,tumor growth,etc. Conclusion：Microtia have obvious lnc RNAs differential expression,these lnc RNAs and certain signaling pathways may play an important role in the occurrence and development of microtia.

关 键 词：先天性小耳畸形 长链非编码RNA 基因芯片 

分 类 号：R764.71[医药卫生—耳鼻咽喉科]