创伤后应激障碍大鼠前额叶皮质PKMζ表达的变化  

The alteration of PKMζ expression in the prefrontal cortex of PTSD rats

作　　者：季丽莉[1] 丁宁[2] 佟雷[1] 彭军波[1] 富长海[1] 徐宝库 王振宇[1] 

机构地区：[1]中国医科大学基础医学院解剖教研室,辽宁沈阳110122 [2]中国医科大学附属第一医院肾内科血液净化室,辽宁沈阳110122

出　　处：《解剖科学进展》2015年第5期501-504,共4页Progress of Anatomical Sciences

基　　金：辽宁省科技厅社会发展攻关计划(No.2012225021);辽宁省自然科学基金项目(No.201102248)

摘　　要：目的探讨创伤后应激障碍(PTSD)大鼠前额叶皮质PKMζ的表达变化情况。方法采用单程长时应激(SPS)制备PTSD大鼠模型,旷场试验、高架十字迷宫试验检测造模效果。将大鼠分为:SPS1d、SPS3d、SPS7d和SPS14d组,正常饲养大鼠作为对照组。利用Western blot和real-time RT-PCR检测PKMζ蛋白及m RNA在大鼠前额叶皮质表达。结果 SPS模型大鼠表现出明显的焦虑症状,SPS7d、SPS14d组大鼠前额叶皮质PKMζ蛋白和m RNA表达水平显著高于对照组。结论在应激后7d和第14d,PTSD大鼠前额叶皮质PKMζ表达显著增高。Objective To investigate the aoteration of PKMζ expression in prefrontal cortex（PFC） of posttraumatic stress disorder（PTSD） rats. Methods The PTSD model was established by single prolonged stress paradigm（SPS）, and identified by Open-field test and elevated plus-maze test. The PTSD rats were divided into： SPS1 d, SPS3 d, SPS7 d and SPS14 d groups, normal fed rats as a control group. The expression of PKMζ protein and mRNA in PFC was detected by Western Blot and real-time RT-PCR respectively. Results Behavioral test results showed that SPS rats had significant anxiety symptom. The expression levels of PKMζ protein and mRNA in prefrontal cortex of SPS7 d and SPS14 d rats were significantly higher compared to control group. Conclusion The overexpression of PKMζ in the PFC of PTSD rats indicates that PKMζ might be involved in the pathogenesis of PTSD.

关 键 词：创伤后应激障碍 前额叶皮质 PKMζ 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]