白及多糖疏水改性后胆甾醇取代度的测定方法  

Determination of Degree of Cholesteryl Substitution in Bletilla Striata Polysaccharide

作　　者：王艳萍[1] 刘福强[1] 刘天娇[1] 刘丹[1] 张琦[1] 

机构地区：[1]解放军208医院药剂科,吉林长春130062

出　　处：《解放军药学学报》2015年第4期285-287,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金项目;No.81173559

摘　　要：目的对白及多糖衍生物胆甾醇取代度的测定方法进行研究。方法采用HPLC法、UV法和核磁共振法(1H-NMR)对合成的胆甾醇琥珀酰基白及多糖进行取代度的测定。结果 HPLC法、UV法、核磁共振法测定胆甾醇取代度的结果分别为3.84%、3.82%、4.26%。结论 3种测定方法都有可行性,HPLC法、UV法比核磁共振法测定胆甾醇值略低,但偏差不大,各有优缺点,可根据对试验结果准确度的要求选择较为合适的测定方法。Objective To compare three methods for determination of the degree of substitution of polysaccha- ride derivatives. Methods High performance liquid chromatography （HPLC）, UV visible spectrophotometric method and nuclear magnetic resonance spectroscopy （NMR） were used to determine the degree of substitution in synthethic cholesteryl succinyl of sugar （CHSB） . Results The degree of substitution were 3. 84%, 3. 82% and 4.26% respectively. Conclusion All the three methods are applicable. HPLC and UV visible speetrophotometry yielded slightly lower values than nuclear magnetic resonance method, but deviation is small. Each has its advantages and disadvantages. The selection of a method depends on the requirements of accuracy of experimental results.

关 键 词：白及多糖 胆甾醇琥珀酰基 取代度合成 理化性质 

分 类 号：R927.2[医药卫生—药学]