肺痨康抗耐异烟肼结核的分子机制与katG基因突变的关系初探  

A Study on Relationships Between the Molecular Mechanism of Feilaokang Capsule Counteracting Isoniazid-resistant Tuberculosis and Its Associated kat G Gene Mutations

作　　者：卢振方[1] 叶品良[1] 张传涛[2] 卢润生[3] 王燕平[1] 陈欢[1] 

机构地区：[1]成都中医药大学,四川成都610075 [2]成都中医药大学附属医院,四川成都610072 [3]四川省疾病预防控制中心,四川成都610041

出　　处：《现代中医药》2015年第5期151-154,185,共5页Modern Chinese Medicine

基　　金：国家自然科学基金资助项目(81173382)

摘　　要：目的初步探讨肺痨康抗耐异烟肼结核杆菌的分子机制与kat G基因突变的关系。方法将耐异烟肼肺结核模型小鼠随机分为空白组、模型组、异烟肼组、肺痨康组、联合用药组,并设标准菌株组。造模成功后,灌胃56 d后,观察并记录小鼠一般体征和体重变化,取出相同部位肺脏组织,HE染色观察小鼠肺组织病理变化,提取部分样本结核杆菌DNA,进行全基因测序,观察kat G基因突变位点变化。结果各治疗组的病理损伤均明显轻于模型组异烟肼组见结核小节形成,肺痨康组及联合用药组未见典型结核结节,全基因测序发现模型组、异烟肼组、肺痨康组以及联合组kat G基因第315、463位密码子由AGC、CGG突变为ACC、CTG,标准菌株未显示kat G基因突变。结论肺痨康可以改善耐异烟肼结核小鼠的生存状态,减少肺组织的炎性反应,其发挥作用的分子机制与kat G基因回复突变相关性较弱。Objective： To preliminarily explore the correlations between the molecular mechanism of Feilaokang Capsule to counteract isoniazid-resistant tuberculosis and katG gene mutations. Methods The isonlazid-resistant tubercu- losis mice were randomly divided into blank, model, isoniazid, Feilaokang and combination groups, with a standard strain group set up. After the model mice created and fed for 56 days, the changes of their body＇ s general signs and weight were observed and recorded. The same parts of the lung tissues were removed to observe their pathological changes by HE stai- ning and take mycobaeterium tuberculosis DNA from part samples for complete gene sequencing to watch the site changes of KatG gene mutation. Results： The pathological damages of each treatment group were significantly lighter than modelgroup and the tubercles were formed in isoniazid group, but there were no typical tuberculous nodules in Feilaokang and combination groups. The complete gene sequencing results showed that the 315th and 463rd codon of katG genes mutated from AGC and CGG into ACC and CTG in model, isoniazid, Feilaokang and combination groups while standard strains did not show katG gene mutations. Conehusion： Feilaokang Capsule can improve the existent state of isoniazid-resistant tu- berculosis mice and reduce the inflammatory reaction of lung tissues. The molecular mechanism of its function has lesser correlations with katG gene reverse mutations.

关 键 词：肺痨康 耐异烟肼结核杆菌 分子机制 KATG基因突变 

分 类 号：R285[医药卫生—中药学] R52[医药卫生—中医学]