RP-HPLC法同时测定忍冬藤中绿原酸、马钱苷、当药苷含量的研究  

Study on Simultaneous Determination of Chlorogenic Acid,Loganin and Chiratin in Lonicera japonica Caulis by RP- HPLC

作　　者：于晓[1,2] 李松涛[2] 刘建升[2] 张永清[3] 李华斌[2] 

机构地区：[1]山东大学药学院,山东济南250355 [2]山东医药技师学院,山东泰安271016 [3]山东中医药大学药学院,山东济南250355

出　　处：《山东农业科学》2015年第9期124-126,145,共4页Shandong Agricultural Sciences

基　　金：国家"十二五"科技支撑计划课题(2011BAI06B01);山东省自然科学基金-青年基金课题(ZR2011HQ057);山东省自主创新专项(2013CXB14001)

摘　　要：采用RP-HPLC法建立了忍冬藤中绿原酸、马钱苷、当药苷三种成分的含量测定方法。色谱条件为：Aglient TC-C18（4.6 mm×250 mm,5μm）色谱柱;流动相：A为乙腈,B为0.4%磷酸溶液,进行梯度洗脱,流速为1.0 m L/min;检测波长为240 nm,柱温25℃。在该色谱条件下,所测定三种成分均达到基线分离,且各成分在线性范围内线性关系良好,精密度、重现性、稳定性均良好,回收率变化范围为98.65%~99.07%。该方法准确、简便、可行,可以用于忍冬藤的质量控制。A new method for simultaneously determining the contents of chlorogenic acid,loganin and chiratin in Lonicerae japonicae caulis was set up by RP -HPLC.The chromatographic conditions were as Aglient TC -C18 column （4.6 mm ×250 mm,5μm）,acetonitrile （A）and 0.4% （V /V）phosphoric acid solution （B）composed as the mobile phase for gradient elution,1.0 mL/min of flow rate,240 nm of detec-tion wavelength and 25℃ of column temperature.The results showed that all the three components were sepa-rated well and their calibration curves showed good linear regression within test ranges;and the precision,re-producibility and stability of this method were better and the recovery rates ranged from 98.65% to 99.07%. In conclusion,this method was accurate,reliable and convenient,so it could be used for the quality evalua-tion of Lonicerae japonicae caulis.

关 键 词：忍冬藤 绿原酸 马钱苷 当药苷 含量测定 

分 类 号：S567.19[农业科学—中草药栽培]