山核桃仁鞣质叶酸-PEG修饰纳米粒的制备和性能研究  

Study on preparation and characterization of tannins in walnut loated folate-PEG nanoparticles

作　　者：李鑫[1] 黄可心[1] 王晓扬[1] 武文强[1] 王立[1] 任君刚[1] 金哲雄[1] 

机构地区：[1]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《功能材料》2015年第20期20016-20019,共4页Journal of Functional Materials

基　　金：黑龙江省教育厅科学技术研究资助项目(12541195)

摘　　要：为了提高山核桃仁鞣质的肿瘤靶向性,增强其抗肿瘤活性,采用薄膜分散法制备山核桃仁鞣质叶酸-PEG修饰纳米粒,通过核磁共振、透射电镜等方法对纳米粒的结构、粒径和粒径分布、形态、稳定性、包封率以及肿瘤靶向性进行了初步研究。结果表明,山核桃仁鞣质叶酸-PEG修饰纳米粒平均粒径为166.8nm;包封率为74.5%,粒子呈大小均匀的球形;放置50d纳米粒粒径和包封率变化轻微,具有很好的稳定性;叶酸-PEG修饰纳米粒小鼠尾静脉注射后0.25,1和4h肿瘤分布分别为20.8%,36.2%和50.2%,具备明显的肿瘤靶向作用。In order to enhance the tumor-targeting and anticancer activity of tannins in walnut,tannin-loaded nanoparticles which decorated by FA-PEG were prepared through film dispersion method.1 H-NMR was employed to characterize the structure of FA-PEG-NH2.The transmitting electron microscope was characterized the morphology of nanoparticles.The average diameter of nanoparticles and size distribution were determined by dynamic light scattering.The drug encapsulation efficiency and tumor-targeting characteristics were determined by HPLC.The results indicated that the nanoparticles were uniform spherical and regular.Its average size was 166.8nm,and encapsulation efficiency was 74.5%.The size and encapsulation efficiency stability of nanoparticles was good because they were little change after 50 d.When the time was 0.25,1,4hafter nanoparticles were injected in tail vein of mice,the distribution of drug concentrations in tumor respectively was 20.8%,36.2%,50.2%.The tumor targeting characteristics of nanoparticles were obvious.

关 键 词：叶酸 PEG 山核桃仁鞣质 纳米粒 肿瘤靶向 

分 类 号：R943.42[医药卫生—药剂学] R979.1[医药卫生—药学]