外源Si对盐胁迫下甘草幼苗形态及生理指标的影响  

Effect of silicon addition on seedling morphological and physiological indicators of Glycyrrhiza uralensis under salt stress

作　　者：崔佳佳[1] 张新慧[2,3,4,5] 李月彤 周达[2] 张恩和[1] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070 [2]宁夏医科大学药学院,宁夏银川750004 [3]宁夏回药现代化工程技术研究中心,宁夏银川750004 [4]宁夏回医药协同创新中心,宁夏银川750004 [5]宁夏医科大学回医药现代化省部共建教育部重点实验室,宁夏银川750004

出　　处：《草业学报》2015年第10期214-220,共7页Acta Prataculturae Sinica

基　　金：宁夏教育厅项目(NGY2012062);国家自然科学基金项目(31260304和31460330)资助

摘　　要：为深入了解Si对盐胁迫下甘草幼苗的影响,本实验采用培养皿滤纸床进行发芽试验,以乌拉尔甘草幼苗为试验材料,在两种浓度Na Cl溶液胁迫下设置不同浓度的硅溶液对甘草幼苗进行处理,通过测定幼苗形态(胚根、胚芽)及生理(质膜相对透性、丙二醛和过氧化物酶)指标,以探讨在盐胁迫下外源硅对甘草幼苗形态及生理指标的影响。结果表明,在Na Cl胁迫下,加入硅(Si)溶液后可以有效增加幼苗胚芽和胚根的长度及重量。在低盐(100mmol/L)胁迫下,Si浓度为0.4 mmol/L时对胚根生长的促进最强;在高盐(200 mmol/L)胁迫下,Si浓度为0.8mmol/L时对胚根生长的促进作用最强。外源Si也不同程度提高了过氧化物酶活性、渗透调节物质脯氨酸含量,以及丙二醛含量和质膜相对透性。由此可见,在盐胁迫条件下,加入适宜浓度的外源硅,可以通过保持一定的抗渗透胁迫能力和清除活性氧的能力,进而保持膜的稳定,来缓解盐胁迫对甘草幼苗生长的抑制作用。In this study,a water culture experiment was conducted to investigate the effect of silicon addition on the morphological indicators( radicle,seed germ) and physiological indicators [membrane permeability,malondialdehyde( MDA) level and peroxidase activities] of Glycyrrhiza uralensis seedlings under salt stress. Two Na Cl concentrations( 100 and 200 mmol/L) and six Si levels( 0,0. 4,0. 8,1. 2,1. 6 and 2. 0 mmol/L) were set as treatments in the experiment. Potassium silicate( K2SO4) was used as the source of Si. The Si addition can effectively increase the length and dry weight of germ and radicle of G. uralensis seedlings under salt stress. Under 100 mmol / L Na Cl,the 0. 4 mmol /L Si treatment promoted radicle growth the most among all the Si treatments,while under 200 mmol / L Na Cl,the 0. 8mmol / L Si treatment promoted radicle growth the most. Exogenous Si addition was also able to increase the peroxidase activity and proline content,and reduce the levels of MDA under salt stress. In conclusion,G. uralensis showed enhanced osmotic stress resistance and reactive oxygen scavenging capacity under salt stress when exogenous Si was added to the culture medium and Si addition alleviated growth suppression of G. uralensis seedlings caused by salt stress.

关 键 词：甘草 硅 盐胁迫 幼苗 生理特性 

分 类 号：S567.71[农业科学—中草药栽培]