二去水卫矛醇对人脑肿瘤细胞体外抑制作用  

Inhibitory Effect of 1,2:5,6-Dianhydrogalctitol on Human Brain Tumor Cells in Vitro

作　　者：梁乔芳 刘华钢[2] 谭强[1] 苏桂玉 彭晓丽[1] 徐红娟[1] 

机构地区：[1]广西中医药大学药学院,广西南宁530001 [2]广西医科大学药学院,广西南宁530021

出　　处：《广西科学》2015年第4期454-456,共3页Guangxi Sciences

基　　金：广西科学研究与技术开发计划项目(桂科能12237007)资助

摘　　要：【目的】探寻二去水卫矛醇(1,2:5,6-Dianhydrogalctitol,DAG)对人脑肿瘤细胞的抑制作用,为DAG抗肿瘤作用提供理论基础。【方法】通过体外实验,并采用噻唑蓝(MTT)比色法检测不同浓度DAG对11株人脑肿瘤细胞的体外抑制作用,观察DAG的半数抑制浓度。【结果】DAG对IMR-32、SH-SY5Y、U87、A172、BT325、T98G、SHG-44、U373、C6、SK-N-BE、U251等11株人脑肿瘤细胞作用72h后半数抑制浓度(72h-IC50)分别为24.28μg/mL、19.24μg/mL、21.55μg/mL、25.13μg/mL、20.63μg/mL、21.03μg/mL、24.91μg/mL、23.52μg/mL、22.06μg/mL、22.92μg/mL、23.44μg/mL。【结论】DAG在体外对上述11株人脑细胞均有抑制生长作用。【Objective】The inhibitory effect of 1,2:5,6-Dianhydrogalctitol(DAG)on 11 strains of human brain tumor cells was investigatedin vitro.【Methods】MTT assay was performed to determine the half inhibitory concentration of DAG for the 11 strains of human brain tumor cells.【Results】The half inhibitory concentration of DAG for IMR-32、SH-SY5 Y,U87,A172,BT325,T98 G,SHG-44,U373,C6,SK-N-BE and U251 within 72hwas 24.28μg/mL,19.24μg/mL,21.55μg/mL,25.13μg/mL,20.63μg/mL,21.03μg/mL,24.91μg/mL,23.52μg/mL,22.06μg/mL,22.92μg/mL and 23.44μg/mL,respectively.【Conclusion】DAG exhibits the inhibitory activity on the above mentioned 11 strains of human brain tumor cells invitro.

关 键 词：二去水卫矛醇 人脑肿瘤细胞 噻唑蓝试验 半数抑制浓度 

分 类 号：R965.1[医药卫生—药理学] Q2-33[医药卫生—药学]