缺氧/复氧对脂多糖诱导人牙周膜成纤维细胞表达环氧合酶-2、前列腺素E_2的影响  

The effect of hypoxia / reoxygenation on the expressions of COX-2 and PGE2in lipopolysaccharide-induced human periodontal ligament cells

作　　者：徐玲玲[1,2] 钱文慧[1,2] 李璐[1,2] 陈旭[1,2] 叶宇[1,2] 徐艳[1,2] 

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室 [2]南京医科大学附属口腔医院牙周科,江苏南京210029

出　　处：《口腔医学》2015年第9期714-717,共4页Stomatology

基　　金：国家自然科学基金资助项目(81170962);国家自然科学基金资助项目(81470749);江苏省高校优势学科建设工程项目(2014-37)

摘　　要：目的观察缺氧/复氧对脂多糖诱导人牙周膜成纤维细胞表达环氧合酶-2、前列腺素E2的影响。方法用1μg/m L脂多糖诱导的人牙周膜成纤维细胞,在1%缺氧浓度下培养6、12、24、48 h,同时分别在缺氧刺激后复氧24 h,用Western Blotting和ELISA检测环氧合酶-2和前列腺素E2的表达。结果缺氧组环氧合酶-2的表达高于对照组,并在24 h达到高峰,复氧组环氧合酶-2的表达较缺氧组和对照组均增加;前列腺素E2的表达在复氧组高于对照组与缺氧组,但在缺氧组和对照组之间无统计学差异。结论缺氧能影响脂多糖诱导人牙周膜成纤维细胞环氧合酶-2的表达,复氧能增强环氧合酶-2和前列腺素E2的表达。缺氧/复氧对环氧合酶-2和前列腺素E2表达的影响在牙周炎的发展中有重要影响。Objective To study the effect of hypoxia / reoxygenation on the the expressions of COX-2 and PGE 2 in lipopolysaccha-ride-inducedind human periodontal ligament cells. Methods Human periodontal ligament cells were first induced by 1 μg / mL lipopo-lysaccharide. Then cells were divided into two experiment groups：hypoxia group and reoxygenation group. In the hypoxia group,cells were cultured in 1% O2 for 6,12,24,48 h,respectively. In the re-oxygenation group,cells were first cultured under the samecondi-tion as the hypoxia group and then returned to normoxic condition for 24 hours. The expression of COX-2 was detected by Wesrtern Blootting and PGE2 was observed by ELISA. Results The expression of COX-2 was higher in the hypoxia group compared to the con-trol group and reached the peak at 24 h. Besides,the expression of COX-2 significantly increased in the reoxygenation group compared to the hypoxia group and the control group. The expression of PGE2 was higher than that in the hypoxia group and the control group. But there was no significant difference between the hypoxia group and the control group. Conclusions Hypoxia could influence the ex-pression of COX-2 in lipopolysaccharide-Induced human periodontal ligament cells but does not affect the expression of PGE2 . Reoxy-genation could increase the expressions of COX-2 and PGE2 . The hypoxia / reoxygenation condition might play an important role in peri-odontitis.

关 键 词：缺氧/复氧 脂多糖 牙周膜 环氧合酶- 2 前列腺素E 2 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]