水稻矮秆多分蘖突变体CA648的农艺性状分析及分子验证  

Agronomic traits analysis and molecular verification of a dwarf multi-tillering rice mutant CA648

作　　者：刘清[1] 朱允华[2] 陈靓靓[1] 李海林[3] 史齐[1] 李合松[1] 沈革志[4] 彭克勤[1] 萧浪涛[1] 

机构地区：[1]湖南农业大学植物激素与生长发育湖南省重点实验室,湖南长沙410128 [2]南华大学药学与生物科学学院,湖南衡阳421001 [3]湖南农业大学农学院,湖南长沙410128 [4]上海市农业科学院作物研究所,上海201106

出　　处：《湖南农业大学学报（自然科学版）》2015年第5期455-461,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(91117006;91317312);湖南省高校创新平台开放基金项目(12K061;09K052);作物种质创新与资源利用重点实验室开放研究项目(12KFXM05)

摘　　要：以T–DNA插入引起的水稻矮秆多分蘖突变体丛矮648(CA648)及对照中花11(ZH11)为材料,进行农艺性状、F2杂交群体性状分离分析及分子验证。结果显示:CA648表现出矮化、多分蘖、花期延迟等突变性状;CA648与ZH11杂交后的F2群体的不同株高统计结果符合单基因控制的1∶3遗传分离比例,F2代材料中所有抗Basta株系均表现出矮化多分蘖特性,且非抗性株系与抗性株系数量之比符合1∶3分离规律;TAIL–PCR等分子试验结合生物信息学分析结果表明,T–DNA插在8#染色体一个功能未知基因LOC_Os08g34258的内部,该基因编码蛋白可能与Subtilisin inhibitor家族蛋白具有类似功能。The rice mutant（CA648） with a T-DNA insertion and a wild-type rice of Zhonghua 11（ZH11） were used to analyze the agronomic and molecular traits in this paper. Compared with ZH11, CA648 displayed a phenotype of dwarfism, multi-tillering and late flowering. In the F2 population, the ratio of thenormal plants to the semi-dwarfing or dwarfing plants was approximately 1∶3. All of the plants resistant to Basta in F2 population were short and multi-tillering. Furthermore, the ratio of non-resistant strains to resistant strains in F2 population is 1∶3. Thermal asymmetric interlaced（TAIL）-PCR analysis and bioinformatics analysis showed that the T-DNA was inserted in the 8thchromosome of an function unknown gene LOC_Os08g34258, which encodes a protein with the function similar to proteins of the subtilisin inhibitor family.

关 键 词：水稻 矮秆 多分蘖 突变体丛矮648 农艺性状 分子验证 

分 类 号：S511.01[农业科学—作物学]