RBP4基因的多态性及其与母猪窝产仔性状的相关性  

Polymorphisms of RBP4 gene and their correlation with sow's litter size

作　　者：昌文林[1,2] 张达军 袁安文[1] 杨青[1] 薛立群[1] 

机构地区：[1]湖南农业大学动物医学院,湖南益阳413046 [2]湖南农业大学动物科技学院,湖南长沙410128 [3]益阳市农科所,湖南益阳413046

出　　处：《湖南农业大学学报（自然科学版）》2015年第5期508-512,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31172377;31272630);湖南省科学技术厅重点项目(2006NK2009);教育部科学技术研究重点项目(212121);湖南省杰出青年项目(2015JJ1009)

摘　　要：基于生物信息学方法,发现并验证了与RBP4基因LS性状(litter size)候选分子标记MspⅠRFLP(SNP G1223C)临近的SNP A1027G(SNPs,single nucleotide polymorphisms),并对大白猪和湖南黑猪群体的RBP4多态性与母猪LS性状的相关性进行了研究。结果表明:RBP4基因SNP G1223C在2个群体中均与母猪LS性状显著相关(P<0.05);RBP4基因SNP A1027G在混合群体中与母猪LS性状显著相关(P<0.05);这2个多态性位点构建的单倍型也与母猪LS性状显著相关(P<0.05),其优势单倍型母畜的LS性状值明显优异于单个SNP位点优势基因型的LS性状值。在分子标记辅助育种中采用RBP4单倍型作为LS性状重要候选基因的分子标记比采用单个多态性位点更具价值。Retinol binding protein 4（RBP4）, an important candidate gene for traits of sow litter size（LS）, plays a key role in vitamin transportation on the maternal-fetal interface during swine pregnancy. Candidates of exonic single nucleotide polymorphisms（SNPs） in gene RBP4 were scanned through bioinformatics analysis, and SNP A1027 G, neighboring to Msp Ⅰ RFLP（SNP G1223C） with molecular marked was validated, and the correlation between LS traits and polymorphisms of RBP4 gene were further analyzed both on the population of Large White and Hunan black. Results exhibited that both RBP4 MspⅠ RFLP and the lately reported SNP A1027 G were significantly correlated with swine LS traits（P〈0.05）. The average LS traits performance of most of advantage RBP4 haplotypes was better than those of advantage genotypes with single locus on SNPs. It was suggested that the haplotype of RBP4 gene was a more efficient molecular marker for the improvement of swine LS traits than SNP with a single locus.

关 键 词：母猪 育种 基因多态性 窝产仔性状 RBP4基因 

分 类 号：S813.3[农业科学—畜牧学]