LCE3C在皮肤癌组织中分布和细胞定位及其调控机制的初步研究  

The LCE3C expression in human skin cancers and the potential regulation mechanisms

作　　者：陈琼琼[1] 涂珍珍[1] 王瑞[1] 张思平[2] 赵盼[1] 成芳[1] 查晓军[1] 周海胜[1] 

机构地区：[1]安徽医科大学生物化学与分子生物学教研室,合肥230032 [2]安徽医科大学附属省立医院皮肤科,合肥230001

出　　处：《安徽医科大学学报》2015年第10期1413-1417,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81172591)

摘　　要：目的研究LCE3C蛋白在皮肤鳞状细胞癌(SCC)以及基底细胞癌(BCC)中的分布及其调控的分子机制。方法免疫组织化学法检测LCE3C在SCC和BCC组织中的表达。构建绿色荧光蛋白融合表达的LCE3C重组表达载体pLCE3C-GFP,转染人SCC细胞系A431,采用激光共聚焦显微镜分析LCE3C在A431细胞中的定位。Western blot法检测转录因子GRHL3对LCE3C表达的影响。结果免疫组化结果显示,与正常皮肤组织比较,LCE3C在SCC和BCC组织中表达明显增加;在皮肤癌组织中LCE3C主要分布在表皮的角质层。激光共聚焦结果显示,LCE3C主要分布在细胞外基质(ECM)中;Western blot结果显示,与角质形成细胞(HaCaT)比较,A431细胞中GRHL3表达量显著降低,而LCE3C表达量明显增高;过量表达GRHL3的细胞(A431/GRHL3)LCE3C表达量显著降低。结论与正常皮肤组织比较,LCE3C在皮肤癌中的表达增加,主要分布在皮肤角质层的ECM;转录因子GRHL3与皮肤癌组织LCE3C的表达呈负相关性。Objective To investigate the LCE3C expresson in squamous cell carcinoma （SCC） and basal cell car-cinoma （BCC）, and the potential mechanisms for regulating LCE3C expression. Methods Immunohistochemical method was performed to detect the expression levels of LCE3 C in the normal skin tissues or the lesion skin tissues from patients with SCC and BCC. To find the cellular localization of the LCE3C proteins, human skin cancer cells were transfected by recombinant plasmid （ pLCE3C-GFP） containing human LCE3C cDNA fused with the GFP, and then were observed under the confocal laser scanning microscopy. To further investigate the potential mecha-nisms for regulating LCE3C expression in skin cancer, Western blot was used to detect the expression levels of GRHL3 and LCE3C in the skin cancer cells. Results Immunohistochemical analysis showed that the LCE3C high-ly expressed at the stratum corneum in the lesion skin tissues from patients with SCC and BCC, compared with the normal skin tissues. LCE3C proteins fused with GFP in A431 cells were mainly observed in extracellular matrix （ ECM） based on the confocal laser scanning. Western blot analysis showed that the expression level of GRHL3 in the A431 cells was reduced than that of the HaCaT cells; on the contrary, the expression level of LCE3C signifi-cantly increased in the A431 cells. Furthermore, LCE3C expression was also significantly decreased in the GRHL3 over-expressed cell （A431/GRHL3） compared with the A431 cells. Conclusion Compared to the normal skin tis-sues, LCE3C is dramatically expressed at the corneous layers in the skin cancer tissues. LCE3C proteins are mainly expressed in ECM, and might be negatively regulated by GRHL3 in A431 cells.

关 键 词：皮肤鳞状细胞癌 基底细胞癌 细胞定位 

分 类 号：R319[医药卫生—基础医学]