JNK通路促进乳化异氟烷引起的大鼠胚胎神经干细胞的增殖抑制  

JNK contributes to the proliferation inhibition of the rat fetal neural stem cells triggered by EI

作　　者：周磊[1] 杨泽勇[1] 李元海[1] 

机构地区：[1]安徽医科大学第一附属医院麻醉科,合肥230022

出　　处：《安徽医科大学学报》2015年第10期1434-1437,1442,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81401279)

摘　　要：目的探讨乳化异氟烷(EI)对原代培养的SD大鼠胚胎神经干细胞增殖的影响以及JNK通路在此影响中的作用。方法建立体外培养胚胎神经干细胞接受EI处理的模型,分为6组(n=8):正常组(N组)、脂肪乳组(F组)、EI处理组(8.12、9.80、12.04 mmol/L EI)、9.80 mmol/L EI组+20μmol/L SP600125组(EISP组)。细胞处理12 h后,采用噻唑蓝(MTT)法分别检测6组胚胎神经干细胞的细胞活力;流式细胞术检测细胞的凋亡率;Western blot法定量检测Caspase-3蛋白表达。实验重复3次。结果与N组比较,F组各项指标无显著差异,EI组细胞增殖的抑制率明显升高(P<0.01),EI组细胞的凋亡率明显升高(P<0.01),EI组明显上调Caspase-3的表达量(P<0.05);EI组内3个浓度之间比较,细胞增殖的抑制率(P<0.01)、细胞的凋亡率以及Caspase-3的表达量差异均有统计学意义(P<0.05);与EI组比较,EISP组细胞增殖的抑制率明显下降(P<0.05),EISP组细胞的凋亡率明显降低(P<0.01),EISP组明显下调Caspase-3的表达量(P<0.05)。结论 EI引起的SD大鼠胚胎神经干细胞的增殖抑制具有一定的浓度依耐性,并且与JNK通路相关。Objective To explore the general anesthetic EI on the rat fetal neural stem cell proliferation（NSCs） and the role played by JNK in the influence. Methods The cultured rat fetal NSCs were randomly divided into 6 groups （n=8 each）：normal group （group N）;fat milk group （group F）;EI groups（including 8. 12,9. 80,12. 04 mmol/L EI） , 9. 80 mmol/L EI group＋20 μmol/L SP600125 group （ group EISP） . After incubated for 12 hours, the cellular effects of EI and cell viability were evaluated by MTT reduction assay. The apoptotic rate of the rat NSCs were determined by flow cytometry, and the expression of protein Caspase-3 was observed by Western blot. Results There was no statistical difference in cell viability, apoptotic rate and protein expression of Caspase-3 in group N and group F. EI group had higher cell apoptotic rate（P〈0. 01）, protein expression of Caspase-3 （P〈0. 05 ） , but lower cell viability than group N （ P〈0. 01 ） . And significant differences were found between three do-ses of EI groups （P〈0. 05）. Compared with EI group, lower cell apoptotic rate （P〈0. 01）, protein expression of Caspase-3 but higher cell viability were observed in group EISP （ P〈0. 05 ） . Conclusion JNK plays an important role in EI-induced cytotoxicity possibly in a dose-dependent manner.

关 键 词：胚胎神经干细胞 乳化异氟烷 增殖抑制 凋亡 

分 类 号：R979.9[医药卫生—药品]