尖锐湿疣患者HPV感染类型和细胞免疫功能的分析  

Analysis of HPV Infection Types and Cellular Immune Function in Patients With Condyloma

作　　者：马晓慧[1] 

机构地区：[1]河南省人民医院检验科,郑州450003

出　　处：《中国继续医学教育》2015年第26期78-80,共3页China Continuing Medical Education

摘　　要：目的研究尖锐湿疣(Condyloma Acuminatum,CA)患者感染的人乳头瘤病毒(Human Papillomavirus,HPV)基因型的状况和分布特点,以及HPV亚型和外周血T淋巴细胞亚群和NK细胞的表达情况,分析尖锐湿疣患者感染人乳头瘤病毒亚型与细胞免疫功能的关联性,为临床治疗CA提供实验依据。方法根据纳入标准,选择81例明确诊断CA患者,治疗前取患者皮疹皮屑通过聚合酶链式反应(PCR)方法检测HPV亚型;在治疗和随访3个月的截止点采血,应用流式细胞仪分析淋巴细胞免疫功能,并与正常人群对照组进行比较。结果单一亚型感染40例(49.38%),混合亚型感染41例(50.62%)。感染患者外周血T细胞与正常人群对照组比较,CD 4+细胞百分比降低,P<0.05,差异具有统计学意义,CD 8+细胞百分比升高,P<0.05,差异具有统计学意义,CD 4+/CD 8+比值低于正常人群,P<0.05,差异具有统计学意义。结论 HPV亚型混合感染和细胞免疫功能异常是促使尖锐湿疣患者感染人乳头瘤病毒的重要因素。Objective To investigate condyloma acuminatum （common sexually transmitted disease, CA ） in patients with human papilloma virus （ human papillomavirus, HPV ） genotype infection status and the characteristics of distribution and HPV subtypes, and peripheral blood T lymphocyte subsets and NK cell expression and clinical treatment of ca provide experimental basis.Methods According to the inclusion criteria, the choice of 81 cases of diagnosed patients with Ca, before treatment take rash dander by polymerase chain reaction （ PCR ） method for detection of HPV subtype; in treatment and followed up for 3 months to cut-off point of blood by lfow cytometry analysis of lymphocyte immune function and with the normal population control groups were compared.Results 40 cases （ 49.38% ） were infected by single subtype and 41 cases （ 50.62%） were mixed subtype. Infection in patients with peripheral blood T cells and＆amp;nbsp;normal control group comparison, the percentage of CD 4＋ cells decreased, P〈0.05, had difference statistically significance, CD 8＋ cell percentage increased,P〈0.05, had difference statistically significance, ratio of CD 4＋/CD 8＋decreased,P〈0.05, had difference statistically significance. Conclusion HPV subtype mixed infection and immune cell function anomaly is prompted condyloma acuminatum patients Infected with human papilloma virus is an important factor.

关 键 词：尖锐湿疣 人乳头瘤病毒 细胞免疫 

分 类 号：R725.53[医药卫生—儿科]