回旋器模拟微重力对白色假丝酵母菌生物学特性的影响研究  

Effects of simulated microgravity using RCCS on the biological features of Candida albicans

作　　者：江文俊[1] 徐冰心[2] 周金莲[3] 黄玉玲[1] 姜福全[1] 李晓鸥[1] 易勇[4] 张建中[3] 崔彦[1] 

机构地区：[1]解放军第306医院普通外科,北京100101 [2]解放军第306医院特种医学实验研究中心,北京100101 [3]解放军第306医院病理科,北京100101 [4]解放军第306医院检验科,北京100101

出　　处：《中华医院感染学杂志》2015年第19期4325-4328,共4页Chinese Journal of Nosocomiology

基　　金：全军医学科研"十二五"重点基金资助项目(BWS11J051)

摘　　要：目的研究三维细胞培养系统(RCCS)对白色假丝酵母菌(CAL)生物学特性的影响,探讨其在模拟微重力环境下的生长规律和耐药性变化,为航天医监医保提供理论依据。方法 2014年2月采用RCCS培养CAL SC5314,试验组8株旋转瓶轴心与地面平行模拟微重力环境,对照组8株即重力组旋转瓶轴心与地面垂直;在试验第7、14和21天,分别应用分光光度计绘制菌株生长曲线、光学显微镜观察菌株形态变化、流式细胞仪检测不同浓度氟康唑(FCZ)作用下的活菌数目。结果与对照组相比,试验组CAL的生长曲线左移,迟缓期缩短,对数生长期和平台期提前,差异有统计学意义(P<0.05);试验组CAL菌丝的生成随模拟失重时相的延长而明显增加;在不同浓度FCZ作用下,试验组的活菌数较对照组明显减少,差异有统计学意义(P<0.05),并且随模拟微重力时相的延长,活菌数不断递减。结论 21d回转器模拟微重力可加速CAL的生长速度,促进菌丝生长,同时能增加菌株对FCZ的敏感性。OBJECTIVE To investigate the effects of simulated microgravity using the rotary cell culture system （RCCS） on biological features of Candida albicans and discuss the growth pattern and changes in drug resistance of C .albicans in the simulated microgravity ,so as to provide theoretical basis for astronautic medical monitoring and protection .METHODS C .albicans SC5314 was cultured in RCCS ,and divided into the experimental group （n=8） which underwent horizontal rotation in RCCS and the control group （n=8） which underwent vertical rotation in RCCS .The growth curve of C .albicans was drawn by using spectrophotometer ,the morphogenic switch of C . albicans was observed by optical microscope ,and the viability of cells exposed to various concentrations of flucon‐azole （FCZ） was assayed by flow cytometry at 7 d ,14 d and 21 d during experiment .RESULTS Compared with the control group ,C .albicans under the modeled microgravity were manifested as the growth curves leftward‐shifted , lag phase shortened ,along with logarithmic phase and stationary phase forwarded （P〈0 .05） .The filamentous forms of the organism were obviously increased in cells in the experimental group along with the prolonging of time phase of simulated microgravity ,compared to the control （P〈0 .05） .The viable count was significantly decreased （P〈0 .05） and the increased time phase of exposure to simulated microgravity resulted in the decrease of viability of cells accordingly in different FCZ concentrations compared to the control .CONCLUSION The study showed that the 21‐day simulated microgravity could increase the growth rate and the filament formation of C .albicans ,mean‐while ,the organism had an increased susceptibility to the antifungal agent FCZ .

关 键 词：模拟微重力一 白色假丝酵母菌 氟康唑 药物敏感性 

分 类 号：R379[医药卫生—病原生物学]