一种鉴别粗品肝素钠不同种属来源的简便方法  

A Simple Method for Identification of Crude Heparin Sodium from Various Species

作　　者：马志华[1] 李志敏[1] 姬胜利[1] 杜旭召[1] 白文举[1] 崔洁[1] 

机构地区：[1]河北常山生化药业股份有限公司,河北石家庄050800

出　　处：《食品与药品》2015年第5期318-323,共6页Food and Drug

摘　　要：目的采用SAX-HPLC快速鉴别不同种属来源的粗品肝素钠。方法通过肝素酶酶解不同种属来源的粗品肝素钠,以其酶解后的二糖组份为考察对象,通过SAX HPLC法比较二糖的相对组成,鉴定种属来源。同时在猪来源肝素中分别添加不同比例(5%,10%,15%,20%,25%)的牛来源肝素或羊来源肝素,经肝素酶酶解后,通过SAX HPLC法鉴别在猪来源肝素中是否能快速检出不同种属来源的其他肝素。结果对于不同种属来源的粗品肝素钠,它们的二糖组成具有显著差异;若猪来源肝素混入5%羊来源肝素,即可通过其二糖组成判断。结论此法操作简便,成本较低,可用于不同种属来源的粗品肝素钠的定性检测。Objective To identify the origin of species of crude heparin sodium by SAX-HPLC. Methods With thedigested disaccharide components as the study object which was got through heparinase hydrolysis of crude heparinsodium, SAX-HPLC was used to identify the origin of species by the comparison of disaccharide relative composition.Meanwhile, bovine-or ovine-sourced heparin was added to the porcine crude heparin at different proportions（5 %, 10 %, 15 %, 20 %, 25 %）. After the digestion by heparinase, it was confirmed whether heparin fromforeign species could be detected quickly within porcine crude heparin by SAX-HPLC. Results There were significantdifferences on disaccharide composition among crude heparin from different species. If porcine crude heparin wasmixed with 5 % ovine crude heparin, it could be detected by the disaccharide composition. Conclusion This method issimple and low-cost, and can be used for the qualitative determination of crude heparin sodium from different species.

关 键 词：粗品肝素钠 二糖组成 SAX-HPLC 种属 

分 类 号：Q538[生物学—生物化学]