RP-HPLC测定橘红化痰丸中苦杏仁苷的含量  

Determination of Amygdalin in Juhong Huatan Wan by RP-HPLC

作　　者：蔡桂萍[1] 司徒伟良 宋粉云[1] 钟兆健[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《食品与药品》2015年第5期360-363,共4页Food and Drug

基　　金：广东省自然科学基金资助项目(5002841)

摘　　要：目的建立反相高效液相色谱法（RP-HPLC）测定橘红化痰丸中苦杏仁苷含量的方法。方法色谱柱为Diamonsil C18;流动相为甲醇-0.1%磷酸水溶液（20：80）,流速1.0 m L/min;检测波长210 nm;柱温30℃。结果苦杏仁苷在5.5-44.0μg/m L范围内有良好的线性关系（r=0.9991）,平均回收率为100.3%,RSD为0.84%（n=6）。结论该法可用于橘红化痰丸中苦杏仁苷的含量测定。Objective To establish a method for determination of amygdalin in Juhong Huatan Wan. Methods A RP-HPLC method was established. The chromatographic column was Diamonsil C1. The mobile phase was a mixture ofmethanol-0.1% phosphoric acid （20：80, v/v）. The flow rate was 1.0 mL/min, and the detection wavelength was 210nm. The column temperature was 30 ℃. Results The linear range of amygdalin was 5.5-44.0 gg/mL （r=0.9991）. Theaverage recovery for amygdalin was 100.3 %, with RSD of 0.84 % （n=6）. Conclusion This method can be used forquantitative determination of amygdalin in the preparation.

关 键 词：橘红化痰丸 苦杏仁苷 反相高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]