冬虫夏草提取液对肾移植大鼠肾缺血再灌注损伤免疫调节作用的影响  

Experimental study of cordyceps sinensis on immune regulation effects to renal ischemia-reperfusion in renal transplantation rats

作　　者：江雪莲[1] 李敏[2] 李莉[4] 郭俐宏[3] 

机构地区：[1]湖北省十堰市太和医院(湖北医药学院附属医院)骨5科,442000 [2]湖北省十堰市太和医院(湖北医药学院附属医院)疼痛科,442000 [3]湖北省十堰市太和医院(湖北医药学院附属医院)针灸科,442000 [4]湖北医药学院机能实验室,十堰442000

出　　处：《国际中医中药杂志》2015年第10期904-908,共5页International Journal of Traditional Chinese Medicine

摘　　要：目的 观察冬虫夏草提取液对同种异体肾移植大鼠肾缺血再灌注损伤的保护作用,探讨其抑制排斥反应的免疫调节作用机制.方法 将 100 只大鼠按随机数字表法分为空白对照组,模型组,环孢菌素组,冬虫夏草小、大剂量组.除空白对照组外,其余各组大鼠进行同种异体左肾移植术.术后2 h,环孢菌素组及冬虫夏草小、大剂量组均腹腔注射环孢菌素A溶液2.0 mg/（kg·d）,冬虫夏草小、大剂量组分别灌胃1%冬虫夏草提取液5、10 ml/kg,空白对照组及模型组腹腔注射10 ml/kg生理盐水.给药后14 d,测定各组大鼠给药前后尿素氮（BUN）、胱抑素C（Cys C）、肌酐（Cr）水平;检测各组大鼠治疗前后总超氧化物歧化酶（T-SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）、总蛋白（TP）、白蛋白（ALB）水平.结果 与环孢菌素组比较,冬虫夏草小、大剂量组大鼠血清 BUN[（8.71±0.87）mmol/L、（9.78±1.01）mmol/L 比（12.59±1.54）mmol/L]、Cys C[（1.36±0.09）mg/L、（1.40±0.11）mg/L比（1.94±0.12）mg/L]、Cr[（117.79± 15.35）μmol/L、（121.28±16.01）μmol/L 比（133.76±17.07）μmol/L]、LDH[（157.32±20.93）U/L、（154.35± 19.39）U/L 比（187.78±25.18）U/L]、MDA[（122.23±19.41）nmol/ml、（125.29±25.97）nmol/ml 比（184.23± 26.74）nmol/ml]水平降低,T-SOD[（80.35±7.71）U/ml、（78.30±7.79）U/ml 比（56.43±7.25）U/ml]水平升高（P〈0.05）.结论 冬虫夏草提取液可抑制肾缺血再灌注造成的脂质过氧化反应、降低MDA及LDH水平、提高T-SOD、TP、ALB水平,减轻肾功能损害,有明显的双向免疫调节作用,与环孢菌素A抗排异反应有协同作用,同时可提高机体免疫力,对同种异体肾移植大鼠肾缺血再灌注损伤具有保护作用。Objective To observe the protective effects of Cordyceps Extract （Cordyceps sinensis, CS） on ischemia reperfusion injury of transplanted kidney in renal allograft rejection, and to investigate the inhibition of immune regulation mechanism.Methods 100 rats were randomly divided into a blank control group, an ischemia reperfusion group, a cyclosporine A group, a Cordyceps high dose group （CSA）, and a Cordyceps small dose group （CSB）. Except the blank control group, all the other groups were underwent same race variant body left renal transplantation. 2 hours after the transplantation, cyclosporin A 2.0 mg/（kg·d） was injected to the cyclosporine group, CSA group, and CSB group. 1% CS of 5 and 10 ml/kg by gavage was respectively given to CSA and CSB groups. 10ml/kg sodium chloride was injected to the bland control group and the ischemia reperfusion group. After 14 days （d） of administration, the content of blood urea nitrogen （BUN）, Cystatin C （Cys C）, creatinine （Cr）, total superoxide dismutase （T-SOD）, serum malondialdehyde（MDA）, lactate dehydrogenase （LDH）, serum total protein （TP）, albumin （ALB） were detected. Results Compared with the cyclosporin A group, the content of BUN（8.71 ± 0.87mmol/L, 9.78 ± 1.01mmol/Lvs.12.59 ± 1.54mmol/L）, Cys C（1.36 ± 0.09 mg/L, 1.40 ± 0.11mg/L vs.1.94 ± 0.12 mg/L）, Cr（117.79 ± 15.35μmol/L, 121.28 ± 16.01μmol/L vs.133.76 ± 17.07 μmol/L）, LDH（157.32 ± 20.93U/L, 154.35 ± 19.39U/L vs.187.78 ± 25.18 U/L）, MDA（122.23 ± 19.41nmol/ml, 125.29 ± 25.97nmol/ml vs.184.23 ± 26.74 nmol/ml） were decreased in the CSA group and CSB group, T-SOD（80.35 ± 7.71U/ml, 78.30 ± 7.79U/ml vs.56.43 ± 7.25 U/ml） was increased（P〈0.05）.Conclusion CS can significantly inhibit lipid peroxidation, renal ischemia reperfusion caused a decrease in MDA and LDH production, increase the activity of T-SOD and TP, ALB, reduce the damage of kidney function, have a dual immunomodulatory effects obvious, with cyclosporin A ant

关 键 词：冬虫夏草提取液 肾移植 再灌注损伤 免疫调节 大鼠 

分 类 号：R692[医药卫生—泌尿科学]