变应性鼻炎患者血清中Eotaxin、ICAM-1、ECP、IL-4、IL-5、IFN-γ的表达水平及临床意义  

Expression and clinical significance of Eotaxin,ICAM-1,ECP,IL-4,IL-5,IFN-γ in the serum of patients with allergic rhinitis

作　　者：陈益丹[1,2] 金肖青[1] 朱杰[3] 王强[3] 房瑛[2] 诸剑芳[1] 

机构地区：[1]浙江医院针灸科,浙江杭州310013 [2]浙江中医药大学,浙江杭州310053 [3]浙江医院检验科,浙江杭州310013

出　　处：《中国卫生检验杂志》2015年第18期3041-3044,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江省中医药管理局重点项目(2010ZZ001);浙江省中西医结合老年医学重点学科项目(2012-XK-A04)

摘　　要：目的检测变应性鼻炎患者血清中Eotaxin、ICAM-1、ECP、IL-4、IL-5、IFN-γ的表达水平,探讨其与变应性鼻炎持续状态及症状的相关性。方法 60例中度、重度变应性鼻炎患者,其中持续性、间歇性各30例,以30例健康体检者作为对照,应用ELISA法对受试者进行血清Eotaxin、ICAM-1、ECP、IL-4、IL-5、IFN-γ水平测定,分析各指标在间歇性与持续性变应性鼻炎患者间的差异性及与症状评分的相关性。结果中度、重度变应性鼻炎患者血清ICAM-1高于对照组,Eotaxin、ECP低于对照组,差异均有统计学意义(P<0.01);IL-4、IL-5、IFN-γ鼻炎组与对照组均在检测敏感水平以下。持续性鼻炎组ICAM-1高于对照组;持续性鼻炎组ECP高于间歇性鼻炎组,差异均有统计学意义(P<0.01)。Eotaxin与间歇性鼻炎组喷嚏症状、持续性鼻炎组鼻塞症状均呈负相关(P<0.05)。结论 Eotaxin、ICAM-1、ECP参与中度、重度变应性鼻炎病理过程,且ECP、ICAM-1与持续状态有关,Eotaxin与喷嚏、鼻塞呈负相关。Objective To evaluate the expression levels of Eotaxin, ICAM - 1, ECP, IL - 4, IL - 5, IFN -γ in serum of pa- tients with allergic rhinitis（AR）, and analyze the correlation among Eotaxin, ICAM -1, ECP, IL- 4, IL- 5, IFN-γ and persistent or intermittent AR and symptom scores. Methods 30 persistent and 30 intermittent AR patients were enrolled into the study, and 30 normal cases were enrolled as the control. Expression levels of serum Eotaxin, ICAM - 1,. ECP, IL - 4, IL - 5, IFN-γ were detected by enzyme- linked immunosorbent assay（ELSIA） method, and analyzed the differences on the inde- xes between the persistent and the intermittent AR patients, and the correlation of symptoms and the scores of clinical symp- toms. Results The level of ICAM - 1 was significantly higher in AR group than that in the control group, while Eotaxin and ECP were significantly lower than those in control group （ P 〈 0.01 ）, the AR group of IL - 4, IL - 5, IFN-γ and the control group were below the detection limits of sensitivity. The level of ICAM - 1 was significantly higher in persistent AR group than in control group. The level of ECP was significantly higher in persistent AR group than in intermittent AR group, and the differ- ence was statistically significant （ P 〈 0.01 ）. The level of Eotaxin was negatively related to sneezing symptoms in intermittent AR patients and nasal congestion symptoms in persistent AR group （P 〈 0.05 ）. Conclusion Eotaxin, ICAM - 1, ECP take part in the pathological process of moderate and severe allergic rhinitis, and the levels of ECP, ICAM -1 were closely related to the duration state. Eotaxin was negatively related to sneezing and nasal congestion symptoms.

关 键 词：变应性鼻炎 嗜酸性粒细胞趋化因子 细胞间黏附分子 嗜酸粒细胞阳离子蛋白 白介素-4 白介素-5 干扰素-γ 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]