表达结核分枝杆菌融合抗原TB10.4-HspX的重组酵母免疫效果研究  

The Study on Mouse Immunoresponses After Vaccination with Saccharomyces cerevisiae Expressing Mycobacterium tuberculosis Fused Antigen TB10.4-HspX

作　　者：张宇飞[1,2] 陆健[1,2] 刘建平[1,2] 王洪海[1,2] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200438 [2]复旦大学遗传工程国家重点实验室,上海200438

出　　处：《复旦学报（自然科学版）》2015年第4期478-483,490,共7页Journal of Fudan University：Natural Science

基　　金："十二五"国家科技重大专项(2012ZX10003008);上海市基础研究重点项目(08JC1401100)

摘　　要：结核病是一种严重危害人类健康的传染病,其防治难点主要在于抗药性菌株的出现以及唯一的抗结核疫苗——卡介苗的效果不理想.为发展新型抗结核疫苗,本研究中构建了表达结核分枝杆菌融合抗原TB10.4-HspX(TB10H)的重组酿酒酵母,分别以皮下注射和滴鼻两种方式免疫小鼠,检测免疫效果.结果显示,该重组酿酒酵母通过皮下注射的方式免疫后刺激小鼠产生TB10H特异性抗体IgG,分泌Th1型细胞因子IFN-γ,而且重组酵母免疫后在一定程度上刺激小鼠树突状细胞的成熟分化和抗原递呈作用.一系列结果表明,表达融合抗原TB10H的重组酿酒酵母能够有效激发小鼠抗原特异性免疫应答.Tuberculosis（TB）keeps to be one of the most fatal infectious diseases in the world. And people are facing great difficulty in TB＇s cure and prevention due to the occurrence of drug-tolerance pathogens and the inefficiency of BCG which is the only available licensed TB vaccine. In this research, a recombinant Saccharomyces cerevisiae expressing Mycobacteriurn tuberculosis fused antigen TB10.4-HspX（TB10H） was constructed and used to vaccinate mouse to evaluate its effect on the immune system. The results showed that the subcutaneous injection vaccination of the recombinant yeast stimulated TB10H-specific IgG production and Thl cytokine IFN-？, secretion. The recombinant yeast can induce the maturation of dendritic cells and increase DCs＇ antigen delivering ability. These results show that the recombinant yeast can induce antigen-Specific immunoresponses in mouse and has the possibility to be developed as an effective vaccine candidate against TB.

关 键 词：结核病 疫苗 重组酿酒酵母 TB10.4 HSPX 

分 类 号：Q36[生物学—遗传学]