三聚氰胺降解菌的分离鉴定及其生物炭固定化研究  

Isolation and Identification of Melamine-degrading Strain and Its Immobilization on Biochar

作　　者：付盼[1] 周辉[2] 赵雨薇[2] 孙明星[2] 沈国清[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海出入境检验检疫局,上海200135

出　　处：《复旦学报（自然科学版）》2015年第4期498-504,共7页Journal of Fudan University：Natural Science

基　　金：国家质检公益性行业科研专项经费(201310269)

摘　　要：从农田土壤中分离筛选出一株能以三聚氰胺为唯一氮源和碳源生长的细菌MB4,经形态学观察、生理生化特征和16SrRNA序列分析,初步鉴定为洋葱伯克霍尔德菌（Burkholderia cepacia）.菌株在10d内对30mg/L三聚氰胺的降解率达到81.67%,其最适生长条件为初始pH7~8,温度32~37℃.动力学研究显示,在底物浓度为10~50mg/L时,米氏方程常数Km=2.545mg/L,最大降解速率为1.24mg/（L·h）.经过35d实际土壤培养后,生物炭固定化降解菌MB4对黑土中50~400 mg/kg三聚氰胺的降解效率可达52.83%~70.33%,其降解动态符合Logistic方程.A high-efficiency degrading strain MtM that used melamine as sole carbon and nitrogen sources was isolated from soil. It was initially identified as Burkholderia cepacia by characteristics of morphological, physiological, biochemical and analysis of 16S rRNA sequence. The strain could degrade 81. 67% o.f 30 mg/L melamine within 10 d. Its optimal growth conditions were determined as follows： primary pH between 7.0 and 8.0, and at 32 to 37 ℃. Thekinetic studies showed that Michaelis constants（Kin） was 2. 545 mg/L, and the maximum degradation rate was 1. 24 rng/（L · h）at the substrate concentration of 10-50 mg/L,. The biochar immobilized strain MB4 could degrade 52. 83%--70. 33% of 50--400 mg/kg melamine in black soil within 35 d, andthe degradation dynamicswere in conformity with Logistic equation.

关 键 词：三聚氰胺 生物降解 生物炭 固定化微生物 土壤 

分 类 号：S143.1[农业科学—肥料学]