NR1受体亚基膜运输与杏仁外侧核长时程增强诱导的关系  

The effect of NR1 receptor trafficking on the induction of LTP in the lateral amygdala in rat

作　　者：高锐 戴承令 于书彦[2] 

机构地区：[1]济南护理职业学院,山东济南250102 [2]山东大学医学院

出　　处：《中国预防医学杂志》2015年第9期666-671,共6页Chinese Preventive Medicine

基　　金：国家自然科学基金项目(81101006)

摘　　要：目的研究NR1受体亚基的运输过程在杏仁外侧核(lateral amygdala,LA)NMDA受体依赖性的长时程增强(LTP)现象诱导中的作用。方法采用膜片钳电生理和细胞生物素标记分析相结合的方法,分别以兴奋性突触后电流(EPSC)和NR1受体亚基在细胞表面的表达量为观察指标,观察NR1受体亚基的运输在LTP诱导过程中的作用。采用高频电刺激(HFS)在杏仁外侧核诱导持续、稳定的LTP。经脑薄片灌流液给予NMDA受体阻断剂D-APV,观察其对LTP诱导过程的影响。然后用生物素标记电刺激诱导LTP前后NMDA受体NR1亚基在神经元细胞膜表面的表达量,观察其膜运输过程与LTP诱导的关系。结果高频电刺激可在杏仁外侧核部位诱导出稳定的LTP现象,EPSCs的幅度比刺激前提高了(49.56±17.23)%(n=8),表现为EPSC的持续增强;经脑薄片灌流液给予D-APV可以阻断LTP的诱导发生,EPSCs幅度为刺激前EPSCs幅度的(103.21±4.13)%(n=6),差异无统计学意义;高频电刺激诱导LTP后神经元细胞表面NR1受体的表面表达量比电刺激前增加了(30.48±5.60)%(n=6),差异有统计学意义。结论 NR1受体的运输过程参与杏仁外侧核部位NMDA受体依赖性的长时程增强现象的诱导。Objective To observe the effect of NR1 receptor on the induction of LTP in the lateral amygdale（LA）. Methods The experiments were performed on brain slices obtained from rat.Excitatory post synaptic currents（EPSCs）were recorded electrophysiologically and the surface expression of NR1 receptor was measured by biotinylation assay.After bathing application of NMDA receptor antagnist D-APV,the induction of LTP was observed. Results HFS（high frequency stimulation）could reliably induce LTP in brain slices,and the amplitude of EPSCs increased significantly by（49.56±17.23）%（P〈0.01,n=8）.Bathing application of D-APV could block the induction of LTP in the lateral amygdala,the amplitude of EPSCs was similar to that of control group（103.21±4.13）%（P〉0.05,n=6）.The surface expression of NR1 receptor increased by（30.48±5.60）%（P0.01,n=6）after the induction of LTP. Conclusions The trafficking of NR1 receptors is involved in the induction of NMDA dependent LTP in the rat lateral amygdala.

关 键 词：杏仁外侧核 NR1受体 长时程增强 受体运输 

分 类 号：Q427[生物学—神经生物学]