片仔癀通过PI3K/Akt信号通路诱导人骨肉瘤U2OS细胞凋亡  

Pien Tze Huang Induces Apoptosis of Human Osteosarcoma Cell Line U2OS via PI3K/Akt Signaling Pathway

作　　者：林海英[1] 刘俊宁[1] 陈炳艺[1] 李楠[1] 张燕[1] 

机构地区：[1]福建中医药大学骨伤学院,福建福州350122

出　　处：《云南中医学院学报》2015年第5期7-11,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81373659)

摘　　要：目的探讨片仔癀诱导骨肉瘤U2OS细胞凋亡与磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)途径的关系。方法采用不同浓度片仔癀(0.4,0.8,1.2mg/m L)作用U2OS细胞,采用MTT法检测片仔癀对U2OS细胞增殖的影响;Hoechst 333258染色观察细胞凋亡情况;Western blot检测PI3K调节亚基p85α(PI3K p85α)、磷酸化PI3K调节亚基p85α(p-PI3K p85α)、蛋白激酶B(Akt)、磷酸化Akt(p-Akt)、核多聚(ADP-核酶)聚合酶(PARP)、裂解PARP(Cleaved PARP)等PI3K/Akt途径相关蛋白的表达。结果片仔癀可明显抑制U2OS细胞的增殖,呈时间和浓度依赖性,诱导U2OS细胞凋亡;Western blot显示p-PI3K p85α、p-Akt蛋白表达明显下调,Cleaved PARP表达量增加,与空白对照组相比有明显差异(P<0.05)。结论片仔癀可通过抑制PI3K/Akt信号途径PI3K p85α、Akt磷酸化,促进PARP裂解,诱导骨肉瘤U2OS凋亡。Objective To investigate the effect of phosphate-dayliositol-3-kinase/protein kinase B（PI3K/Akt） signaling pathway on Pien Tze Huang induced apoptosis of human ostesosarcom cell line U2 OS. Methods U2 OS cells were treated by different concentrations of Pien Tze Huang（0.4,0.8,1.2mg/m L）. The anti-proliferative effect of Pien Tze Huang on U2 OS cells was measured by MTT. The apoptosis was detected by Hoeschst 33258 staining. The expression levels of the regulatory subunit of PI3 K p85α（PI3K p85α）、phospho-PI3 K p85α（p-PI3 K p85α）、protein kinase B（Akt）、phospho-Akt（p-Akt）、Poly（ADP-ribose）polymerase（PARP）、the cleavage of PARP（Cleaved PARP） were analyzed by Western blot. Results Pien Tze Huang suppressed the proliferation of U2 OS cells in a time and dose-dependent manner. Topical morphological changes were observed by Hoechst 33258 staining. The expressions of p-PI3 K p85α、p-Akt were reduced and Cleaved PARP expression was increased while indicating a statistically significant difference as compared with the blank control group（P〈0.05）. Conclusion Pien Tze Huang induces apoptosis of human osteosarcoma cell line U2 OS through inhibiting PI3 K p85αand Akt phosphorylation in PI3K/Akt signaling pathway and increasing the expression of Cleaved PARP.

关 键 词：片仔癀 骨肉瘤 PI3K/AKT信号通路 凋亡 

分 类 号：R285.5[医药卫生—中药学]