响应面法优化三叶青总黄酮的提取工艺研究  

Optimization of Total Flavonoids Extraction from Radix Tetrastigmae by Response Surface Methodology

作　　者：卢舜飞[1] 汪亮亮[2] 童应鹏[3] 叶挺梅[2] 

机构地区：[1]浙江丽水学院医学与健康学院,丽水323000 [2]浙江丽水学院生态学院,丽水323000 [3]浙江工业大学药学院,杭州310014

出　　处：《海峡药学》2015年第9期34-38,共5页Strait Pharmaceutical Journal

基　　金：浙江省自然科学基金项目(LQ12H29001);浙江省自然科学基金项目(LQ14H290001);浙江省科技厅重大科技专项社会发展项目(2011C03002)

摘　　要：目的优选三叶青总黄酮的提取工艺。方法以液料比、乙醇浓度、提取时间、提取温度4个单因素为考察基础,利用响应面法优化并建立了三叶青总黄酮最佳的回流提取工艺。结果液料比对总黄酮提取量的影响最大,提取时间次之。最佳工艺条件为:液料比14.25m L·g-1,乙醇浓度:50.7%,提取时间:1.975h,提取温度:89.4℃。结论采用响应面分析法优选三叶青总黄酮的提取工艺稳定、合理、可行。OBJECTIVE To optimize the extraction technics of total flavonoids from Radix Tetrastigmae. METHODS Based on single factor experiments, extraction conditions includ liquid-solid ratio, ethanol con- centration, extraction time and extraction temperature were optimized using response surface methodology. RESULTS The main factor of influencing total flavonoids extraction was liquid-solid ratio followed by extraction time. The optimal extraction conditions were as follows：liquid-solid ratio 14. 25mL · g^- 1, ethanol concentration 50. 7 %, extraction time 1. 975h and extraction temperature 89. 4℃. CONCLUSION Optimization of total flavonoids extraction from Radix Tetrasdgmae by response surface methodology was steady,reasonable and practicable.

关 键 词：三叶青 总黄酮 提取 响应面 

分 类 号：TQ460.4[化学工程—制药化工]