颠茄合剂中托烷类生物碱的定性与定量研究  

Qualitative and Quantitative Research of the Tropane Alkaloids in Inbelladonna Mixture

作　　者：张满常 尹兆姣 张伦 

机构地区：[1]云南省保山市食品药品检验所,保山678000 [2]云南省中医学院,昆明650000

出　　处：《海峡药学》2015年第9期61-63,共3页Strait Pharmaceutical Journal

摘　　要：目的对颠茄合剂中托烷类生物碱进行定性与定量研究。方法利用反相高效液相色谱法对颠茄合剂中硫酸阿托品、东莨菪碱及山莨菪碱进行定性鉴别,同时对硫酸阿托品进行含量测定。色谱条件：色谱柱：ZORBAX SB-C18（4.6×150mm,5μm）;流动相：乙腈-磷酸盐缓冲液（取磷酸二氢钾6.8g溶于1000mL水中,加三乙胺10mL,调p H至2.8）（13∶87）,检测波长：210nm;流速为1m L·min^-1,进样量20μL,柱温：30℃。结果样品溶液色谱图中显示与对照品溶液色谱图中相同的色谱峰。硫酸阿托品在15.06-120.5μg·m L^-1范围内呈良好线性关系（r=0.9998）;平均回收率（n=9）为98.27%,RSD为0.85%。结论该方法准确、可靠、专属性强,为建立颠茄合剂质量标准提供依据。OBJECTIVE To establish a method for qualitative and quantitative determination of the trapane al-kaloids in Inbelladonna mixtuxe.METHODS RP-HPLC methods was adopted for the atropine , scopolamine and anisodamine identification and the content of Atropine sulfate was also determined.The analyses were performed on a ZORBAX SB-C18（4.6 ×150mm,5μm） column with the mobile phase consisted of acetonitrile-phosphate buffer （pH 2.8）（13∶87）at a flow rate of 1.0mL·min^-1,the detection wavelength was 210nm,the temperature of column was 30℃,and the injection volume was 20μL.RESULTS The chromatogram of the sample solution showed similarity to the reference solution.The linear range of Atropine sulfate was in the range of 15.06 -120.5μg · mL^ -1 （ r =0.9998）,the average recovery（n=9） was 98.27%and the RSD was 0.85%.CONCLUSION The method is ac-curate,reliable and specific ,which can be used for the quality contorl of Belladonna mixture.

关 键 词：颠茄合剂 硫酸阿托品 东莨菪碱 山莨菪碱 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]