MAP洗涤液应用于自体血回收对红细胞形态影响的体外观察  

Observation of the effect of MAP used in autologous blood recovery on the morphology of RBC in vitro

作　　者：戴光艳 吴祥[2] 张峰 李超芬 徐义国[2] 

机构地区：[1]宁波市小港医院,浙江宁波315800 [2]宁波大学医学院附属医院 [3]宁波市第九医院

出　　处：《中国输血杂志》2015年第9期1087-1089,共3页Chinese Journal of Blood Transfusion

摘　　要：目的探讨MAP洗涤液应用于自体血回收及体外保存对RBC形态的影响。方法选择18名择期施行关节或者脊柱手术的患者,随机分为MAP组和生理盐水组,分别采用MAP液和生理盐水术中自体血回收洗涤,术后将回收的RBC和同患者的血浆混匀,在37.0℃、持续摇摆的状态下保存。分别在保存即刻、保存12 h及24 h检测此血样的RBC形态。结果随着保存时间的延长,2组RBC形态均逐渐变化,但MAP组棘形细胞比例(21.5±13.5)%低于生理盐水组(42.1±16.6)%,差异有统计学意义(P<0.05)。结论在模拟体外环境下,随着保存时间的延长,2组RBC形态均逐渐变化,但使用MAP液作为自体血回收洗涤液更有利于维持RBC的形态。Objective To investigate the application and effect of MAP used in autologous blood recovery and in vitro preservation on RBC morphology. Methods 18 patients undergoing selective implementation of joint or spine surgeries were randomly divided into MAP group and saline group. MAP and saline were used respectively in each group during intraopera- tive autologous blood collection. Recycled autologous RBCs and patient plasma were mixed at 37.0℃ and preserved under the condition of continuous fluctuation after surgeries. Twelve and twenty four hours immediately after preservation, RBC morphology was investigated. Results With the prolonging of storage time, the morphologies of washed RBCs after preservation 12 h and 24 h began to differ gradually. The acanthocyte ratio in MAP group was lower than the saline group. The difference showed statistical significance （ P 〈 0.05 ）. Conclusion The morphologies of RBCs in two groups gradually changed as time prolonged, under the Simulation environment in vitro. Application of the MAP for autologous blood recovery is more advantageous to maintain the shape of the RBCs.

关 键 词：MAP液 自体血回收 RBC形态 体外保存 

分 类 号：R457.1[医药卫生—治疗学]