用Bicine-NaOH缓冲液制备的尿酸酶复合纳米脂质体中尿酸酶的生化特性  

Preparation and property of uricase in uricase-catalase Bicine-NaOH nanoliposomes

作　　者：周云莉 何丹[1] 张敉[1] 冯娇[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心,重庆400016

出　　处：《华西药学杂志》2015年第5期543-546,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:30973645)

摘　　要：目的研究Bicine-Na OH缓冲液制备的尿酸酶(UCA)-过氧化氢酶纳米脂质体(UCNL)中UCA的生化特性及初步稳定性。方法使用Bicine-Na OH缓冲液并采用逆向蒸发法制备UCNL,考察UCNL的形态结构、粒径分布、Zeta电位、包封率及UCNL中UCA的最适温度、最适p H、米氏常数和热稳定性、贮存稳定性、酸碱稳定性、抗胰蛋白酶水解能力和抗部分金属离子和有机化合物的能力。结果 UCNL呈类圆形,包封率为56.87%±3.81%,粒径为759.30±28.12 nm,Zeta电位为-8.49±3.18 m V。UCNL中UCA和游离UCA的最适温度均为40℃,最适p H分别为8.0、8.5,Km值分别13.40、13.87μmol·L-1;UCNL中UCA的各种稳定性均比游离UCA好。结论 UCNL中UCA的催化活性和稳定性较游离酶明显增加。OBJECTIVE To investigate tile biochemical characteristics and stabilities of uricase（UCA） loaded in uricase - catalase nannliposomes（UCNL） which were produced by Bicine - NaOH buffer solution. METHODS UCNL were prepared using rcverse - phase evaporation,in which Bicine - NaOH buffer was used. Its morphology, size distribution,Zeta potential and encapsulation efficiency were investigated. Optimum temperature, optimum pH, michaelis - menten constand, thermal stabilities, storage stabilities, pH stabili- ties,stabilities to proteolytic digestion and stabilities to several metal ions and organic compounds of UCA in UCNL and free UCA were primarily examined. RESULTS The shape of UCNL was similar round. The average entrapment efficiency of UCNL was 56.87% ± 3.81%. Tbe particle size of UCNL was 759.30 ± 28.12 nm, and the Zeta potential was - 8.49 ± 3. 18 inV. Optimum temperature of UCA in UCNL and free UCA was 40 ℃ , and optimum pH value of UCA in UCNL and free UCA were 8.0 and 8.5, respectively. Mictmelis - menten constand of UCA in UCNL and free UCA were 13.40 μmol·L^-1 and 13.87 μmol·L^-1. Various stabilities of UCA in UCNL were better than free UCA. CONCLUSION The activity and stabilities of UCA in UCNL are better than free UCA.

关 键 词：尿酸酶 过氧化氢酶 纳米脂质体 生化特性 稳定性 包封率 逆向蒸发法 

分 类 号：R94[医药卫生—药剂学]