具有分子识别特性的新型大黄酸功能化树脂的制备  

Preparation of rhein-functionalized resin with the molecular recognition characteristics

作　　者：谢静[1,2] 青芳竹 青琳森[3] 黄静[2] 

机构地区：[1]成都医学院,四川成都610500 [2]四川大学华西药学院,四川成都610041 [3]中国科学院成都生物研究所,四川成都610041

出　　处：《华西药学杂志》2015年第5期547-549,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:21202161);中国博士后科学基金第6批特别资助(No.2013T60863);2015年四川省大学生创新项目(No.201513705078);四川省卫生厅科研课题(No.120497)

摘　　要：目的合成一种具分子识别特性的新型分离材料大黄酸功能化树脂。方法将大黄酸通过酰氯中间体键合到氨基树脂表面,制备新材料并表征,用所制备的新型功能化树脂填装SPE柱,对虎杖提取液中的蒽醌类成分进行固相萃取,并用HPLC/MS法检测结果。结果通过简单的吸附-解吸附过程,虎杖中的大黄素、大黄素甲醚、芦荟大黄素被选择性富集出来,对大黄素的富集最显著。结论大黄酸功能化的树脂可有效地富集蒽醌类化合物,操作简便、选择性好、富集效率高。OBJECTIVE To synthesize a novel separation material - rhein - functionalized resin with molecular recognition ability. METHODS The rhein - functionalized resin was synthesized by condensation of the acyl chloride intermediate of rhein with aminom- ethyl polystyrene resin, and then characterized by a variety of instruments. And then a rhein - functionalized resin - based solid - phase extraction experiment coupled with HPLC - MS analysis was carried out simultaneously to test the absorbability to anthraquinones in Polygonum cuspidatum extracts. RESULTS Emodin,chrysophanol and physcion were selectively concentrated from low concentration via a simple absorption and desorption procedure, and the concentration of emodin was more obvious. CONCLUSION The rhein - functionalized resin could effectively improve the enrichment of anthraquinones with characteristics of simple operation, high selectivity and high efficiency of enrichment.

关 键 词：分子识别 大黄酸 功能化树脂 新型分离材料 蒽醌 

分 类 号：R94[医药卫生—药剂学]