UPLC测定沙棘膏中的3种黄酮类成分  

Determination of three flavonol aglycones in Seabuckthorn pastes by UPLC

作　　者：杜蕾蕾[1] 吴燕[2] 陈雏[2] 刘瑛[3] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]四川省中医药科学院,四川成都610041 [3]成都大学医护学院,四川成都610106

出　　处：《华西药学杂志》2015年第5期595-597,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81202908);四川省教育厅科研项目(No.14ZB0082)

摘　　要：目的采用UPLC法同时测定藏药沙棘膏中槲皮素、山柰酚和异鼠李素的含量。方法色谱柱为Waters BEH C18柱（50 mm×2.1 mm,1.7μm）,流动相为乙腈-0.1%磷酸（25∶75）,流速0.4 m L·min-1,柱温35℃,检测波长370 nm。结果槲皮素、山柰酚、异鼠李素的线性范围分别为0.75-22.4 ng（r=0.9999）、0.43-12.8 ng（r=0.9996）、0.83-24.9 ng（r=0.9998）;平均加样回收率分别为95.4%、98.7%、96.5%（n=9）。结论所用方法准确、简便,可用于沙棘膏的质量评价,不同沙棘膏中黄酮类成分的含量差异较大,可能来源于不同沙棘属植物的果实。OBJECTIVE To determine the contents of quercetin, kaempferol and isorhamnetin in Seabuckthorn pastes by UPLC. METHODS A Waters BEH C18 column（50 mm ×2.1 mm,1.7μm）was used at temperature 35℃. The mobile phase consisted of acetonitrile and 0. 1% phosphoric acid（25 ： 75）with the flow rate of 0.4 mL·min-1 ,and the detection wavelength was set at 370 nm. RESULTS The linear ranges of quercetin, kaempferol and isorhamnetin were 0.75 - 22.4 ng （ r = 0. 9999 ）, 0.43 - 12.8 ng （ r =0. 9996） and 0. 83 - 24.9 ng （ r = 0. 9998 ）, and their average recoveries were 95.4%, 98.7% and 96.5 % （ n=9 ）, respectively. CONCLUSION The method is simple, accurate and applicable for the qualititative evaluation of Seabuckthorn pastes. There are significant differences of flavonoids contents among the different Seabuckthorn pastes which may be made from different seabuckthorn berries.

关 键 词：沙棘膏 藏药 黄酮 槲皮素 山柰酚 异鼠李素 超高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]