K-Domain Splicing Factor OsMADS1 Regulates Open Hull Male Sterility in Rice  

作　　者：SUN Lian-ping ZHANG Ying-xin ZHANG Pei-pei YANG Zheng-fu ZHAN Xiao-deng SHEN Xi-hong ZHANG Zhen-hua HU Xia XUAN Dan-dan WU Wei-xun LI Zi-he CAO Li-yong CHENG Shi-hua 

机构地区：[1]National Center for Rice Improvement / Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute [2]College of Agronomy, Shenyang Agricultural University

出　　处：《Rice science》2015年第5期207-216,共10页水稻科学（英文版）

基　　金：supported by the National Key Technology R&D Program of China (Grant No.2011BAD35B02);the National Key Transform Program of China (Grant No.2011ZX08001-002);Zhejiang Provincial Natural Science Foundation of China (Grant No.LQ14C130003);the Super Rice Breeding Innovation Team and Rice Heterosis Mechanism Research Innovation Team of the Chinese Academy of Agricultural Sciences Innovation Project, China (Grant No.CAAS-ASTIP2013-CNRRI)

摘　　要：We identified the rice floral organ development mutant, termed as open hull and male sterile 1 （ohms1）, from the progeny of the indica restorer line Zhonghui 8015 treated with 60Co γ-ray irradiation. The ohms1 mutant exhibited an open hull and lemma- and palea-like structure conversion between the anthers and stigma, which resulted in the ohms1 mutant spikelet showing‘tridentate lemma＇. The ohms1 mutant was entirely sterile but had 60%-70% fertile pollen. Genetic analysis and gene mapping showed that ohms1 was controlled by a single recessive gene, and the mutant gene was fine-mapped to a 42-kb interval on the short arm of chromosome 3 between markers KY2 and KY29. Sequence analysis of the four open reading frames in this region revealed that the mutant carried a single nucleotide transformation （A to G） at the last base of the fifth intron, which was likely corresponded to ohms1 phynotype, in an MIKC type MADS-box gene OsMADS1 （LOC_Os03g11614）. Enzyme digestion and cDNA sequencing further indicated that the variable splicing was responsible for the deletion of the sixth exon in ohms1, but no structural changes in the MADS domain or amino acid frame shifts appeared. Additionally, real-time fluorescent quantitative PCR analysis showed that the OsMADS1 expression level decreased significantly in the ohms1 mutant. The expression levels of rice flowering factors and floral glume development-related genes also changed significantly. These results demonstrate that OsMADS1 may play an important role in rice floral organ development, particularly in floral glume development and floret primordium differentiation.We identified the rice floral organ development mutant, termed as open hull and male sterile 1 （ohms1）, from the progeny of the indica restorer line Zhonghui 8015 treated with 60Co γ-ray irradiation. The ohms1 mutant exhibited an open hull and lemma- and palea-like structure conversion between the anthers and stigma, which resulted in the ohms1 mutant spikelet showing‘tridentate lemma＇. The ohms1 mutant was entirely sterile but had 60%-70% fertile pollen. Genetic analysis and gene mapping showed that ohms1 was controlled by a single recessive gene, and the mutant gene was fine-mapped to a 42-kb interval on the short arm of chromosome 3 between markers KY2 and KY29. Sequence analysis of the four open reading frames in this region revealed that the mutant carried a single nucleotide transformation （A to G） at the last base of the fifth intron, which was likely corresponded to ohms1 phynotype, in an MIKC type MADS-box gene OsMADS1 （LOC_Os03g11614）. Enzyme digestion and cDNA sequencing further indicated that the variable splicing was responsible for the deletion of the sixth exon in ohms1, but no structural changes in the MADS domain or amino acid frame shifts appeared. Additionally, real-time fluorescent quantitative PCR analysis showed that the OsMADS1 expression level decreased significantly in the ohms1 mutant. The expression levels of rice flowering factors and floral glume development-related genes also changed significantly. These results demonstrate that OsMADS1 may play an important role in rice floral organ development, particularly in floral glume development and floret primordium differentiation.

关 键 词：RICE open hull male sterile ohms1 gene mapping alternative splicing floral organ 

分 类 号：S511[农业科学—作物学] S512.103.5