不同产地枳壳挥发油成分的气相色谱-质谱分析及抗肿瘤活性研究  

GC-MS Analysis of Volatile Oil Components in Fructus Aurantii from Different Regions and Study of Its Antitumor Activity

作　　者：邢娜[1] 舒尊鹏[1] 徐炳清 焦文娟[3] 李振宇[4] 王秋红[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室黑龙江中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040 [2]齐齐哈尔市中医医院,黑龙江齐齐哈尔161000 [3]江苏康缘药业股份有限公司,江苏连云港222000 [4]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药信息》2015年第5期1-6,共6页Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划"973"项目(No.2013CB531800);国家自然科学基金项目(No.81473351);黑龙江省教育厅科学技术研究项目(No.12521516):黑龙江中医药大学优秀创新人才支持计划(2012)

摘　　要：目的:提取不同产地及炮制方法的枳壳挥发油成分,对其进行化学成分分析,并筛选枳壳挥发油的抗肿瘤活性。方法:使用麦麸对枳壳进行炮制,采用2010年版《中国药典》附录XD甲法提取枳壳挥发油并通过气相色谱-质谱联用法(GC-MC)分析,用面积归一化法计算各成分的相对百分含量;再分析枳壳炮制品挥发油的主要成分,最后用四甲基偶氮唑蓝(MTT)比色法研究枳壳挥发油的抗肿瘤活性。结果:共鉴定93个化学成分,其中柠檬烯是枳壳挥发油中的主要成分,且江西樟树产地的含量最多,同时发现炮制后其含量增加。江西樟树的枳壳挥发油对肝癌细胞(BEL-7402)、胃癌细胞(SGC-7901)、肺癌细胞(SPCA-1)具有不同程度的抑制活性,且呈剂量依赖性,同时发现炮制后的枳壳挥发油抑制效果更好。结论:通过GC-MC方法鉴定不同产地的枳壳中挥发油的化学成分,其主要为柠檬烯,炮制品的柠檬烯含量升高。江西樟树的枳壳挥发油对肝癌细胞(BEL-7402)、胃癌细胞(SGC-7901)、肺癌细胞(SPCA-1)的生长具有一定程度的抑制作用,且应用炮制品的效果更好,这为枳壳的开发利用提供了理论依据。Objective：To study the chemical constituents and antitumor activity of volatile oil from Fructus au- rantii. Methods ： The Fructus aurantii was prepared by stir - baked with bran. The volatile oil was extracted byGC -MS from China pharmacopoeia appendix XD method（2010 edition）. The relative content of each compo- nent was calculated by area normalization method. Moreover, analyze the main components of the volatile oil in processed pieces and study the volatile oil from Fructus aurantii on antitumor activity with tetramethyl azo thia- zole blue （MTr） calorimetric method. Results ： Ninety - three chemical components were identified. Limonene was the most prominent component, and its relative content was the highest in Zhangshu region of Jiangxi prov- ince and increased in the processed products. The volatile oil from Zhangshu region showed different degrees of inhibitory activity against liver cancer ceils （ BEL - 7402）, gastric cancer cell （ SGC - 7901 ） and lung car- cinoma cell （ SPCA - 1 ） and in dose - dependent manner, the inhibitive effect of volatile oil from processed Fructus aurantii was better. Conclusion ： By GC - MC method we can identify volatile oil of Fructus aurantii in different regions, and the main component is limonene and its relative contents increase in the processed prod- ucts. The volatile oil from Zhangshu region has a good inhibitory effect on the proliferation of liver cancer cells （ BEL - 7402 ）, gastric cancer cell （ SGC - 7901 ） and lung carcinoma cell （ SPCA - 1 ） in vitro and the effect of application of the processed products is better. The analytic results can provide theoretical basis for the fur- ther exploitation and utilization of Fructus aurantii.

关 键 词：枳壳 挥发油 化学成分 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]