牛膝各化学拆分组分的含量测定  

Determination of Contents of Split Fractions of Radix Achyranthis Bidentatae

作　　者：张颖[1] 杨柳[1] 张妍妍[1] 姜海[1] 杨炳友[1] 王秋红[1] 肖洪彬[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040

出　　处：《中医药信息》2015年第5期50-53,共4页Information on Traditional Chinese Medicine

基　　金：黑龙江中医药大学优秀创新人才支持计划项目(2012);黑龙江省博士后基金项目(No.LBH-Z10020);黑龙江中医药大学博士创新基金项目(No.2013bs04);黑龙江省自然科学基金面上项目(No.H201324);高等学校博士学科点专项科研基金新教师类项目(No.20132327120003)

摘　　要：目的：建立紫外分光光度法测定牛膝各化学拆分组分中总甾酮和总皂苷含量的方法。方法：采用紫外分光光度法以β-蜕皮甾酮和人参皂苷R0为对照品,在最大吸收波长处分别测定。结果：β-蜕皮甾酮248nm处有最大吸收,在5.44~32.64μg/m L范围内呈良好线性关系（r=0.998）,平均回收率分别为101.72%（n=6）。人参皂苷R0显色后在560nm处有最大吸收,在45.2~271.2μg范围内呈良好线性关系（r=0.997）,平均回收率分别为104.31%（n=6）。结论：本方法可以快速准确的测定牛膝各化学拆分组分中总甾酮和总皂苷的含量。Objective： To establish a UV spectrophotometry method for the determination of total sterones and total saponins in split fractions of radix achyranthis bidentatae. Methods： Use ultraviolet spectrophotometer to measure total sterones and total saponins contents, β -ecdysone and ginsenoside R0 as reference substances, at the maximum absorption wavelength, respectively. Results： β -ecdysone with maximum absorption at 248nm, it had a good linear relationship within the range of 5.44 μg/mL -32. 64μg/mL （r = 0.998 ）, and the average recovery was 101.72% （n = 6）. Ginsenoside R0 with maximum absorption at 560nm, it had a good linear relationship within the range of 45. 2μg - 271.2μg （ r = 0. 997 ）, and the average recovery was 104. 31% （n =6）. Conclusion： The method is simple, accurate, and can be used for the quantitative deter- mination of total sterones and total saponins.

关 键 词：牛膝 甾酮 皂苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]