HPLC梯度洗脱法同时测定保安万灵丹中升麻素苷、5-O-甲基维斯阿米醇苷、茅术醇、β-桉叶醇和苍术素的含量  

Simultaneous Content Determination of Prim-O-Glucosylcimifugin, 5-O-Methylvisammioside, Hinesol, β-Eudesmol and Atractylodin in Bao'an Wanling Pills by Multiwavelength HPLC

作　　者：杨雪梅 班小军 杨海红 范潇予 

机构地区：[1]甘肃省金昌市药品检验检测中心,金昌737100 [2]甘肃省张掖市食品药品检验检测中心,张掖734000 [3]沈阳药科大学中药学院,沈阳110013

出　　处：《中国药事》2015年第9期962-967,共6页Chinese Pharmaceutical Affairs

摘　　要：目的：建立同时测定保安万灵丹中升麻素苷、5-O-甲基维斯阿米醇苷、茅术醇、β-桉叶醇和苍术素含量的方法。方法：采用高效液相梯度洗脱法,色谱柱为C18色谱柱（200 mm×4.6 mm,5μm）,流动相A为甲醇-乙腈（2∶1）,流动相B为0.1%磷酸水溶液,体积流量1.0 m L·min-1,检测波长分别为254 nm（升麻素苷和5-O-甲基维斯阿米醇苷）、203 nm（茅术醇和β-桉叶醇）和340 nm（苍术素）。结果：5种成分线性范围分别为升麻素苷4.30~86.00μg·m L-1（r=0.9994）、5-O-甲基维斯阿米醇苷5.85~117.00μg·m L-1（r=0.9997）、茅术醇16.42~328.40μg·m L-1（r=0.9993）、β-桉叶醇15.16~303.20μg·m L-1（r=0.9999）、苍术素12.78~255.60μg·m L-1（r=0.9995）;精密度、重复性及稳定性试验RSD〈2.0%;平均加样回收率（n=6）分别为升麻素苷97.49%（RSD为1.45%）、5-O-甲基维斯阿米醇苷96.81%（RSD为0.94%）、茅术醇98.60%（RSD为1.65%）、β-桉叶醇97.96%（RSD为1.38%）、苍术素99.22%（RSD为1.24%）。结论：该方法操作简便,结果准确,重复性好,可作为保安万灵丹的含量控制方法。Objective： To develop a method for the simultaneous content determination of prim-oglucosylcimifugin, 5-o-methylvisammioside, hinesol, β-eudesmol and atractylodin in Bao’an Wanling pills. Methods： The gradient elution HPLC method was adopted. The quantitative analysis was carried out on the C18 column（200 mm×4.6 mm, 5 μm）. A linear elution of acetonitrile-methanol（2 ∶ 1） and 0.1% phosphoric acid solution were adopted as the mobile phase at the fl ow rate of 1.0 m L·min-1. The detection wavelength was 254 nm for prim-o-glucosylcimifugin and 5-o-methylvisammioside, 203 nm for hinesol and β-eudesmol, 340 nm for atractylodin. Results： The linear ranges of prim-o-glucosylcimifugin, 5-o-methylvisammioside, hinesol, β-eudesmol and atractylodin fell within the ranges of 4.30-86.00 μg·m L-1（r=0.9994）, 5.85-117.00 μg·m L-1（r =0.9997）, 16.42-328.40 μg·m L-1（r=0.9993）, 15.16-303.20 μg·m L-1（r=0.9999）, and 12.78-255.60 μg·m L-1（r =0.9995）, respectively. RSDs for precision, stability and repetition tests were all 〈 2.0%. The average recoveries and RSDs were 97.49%（1.45%）, 96.81%（0.94%）, 98.60%（1.65%）, 97.96%（1.38%） and 99.22%（1.24%） for the above components, respectively. Concolusion： The method is simple, accurate, and reproducible and can be used in the simultaneous content determination of prim-o-glucosylcimifugin, 5-o-methylvisammioside, hinesol, β-eudesmol and atractylodin in Bao’an Wanling pills.

关 键 词：保安万灵丹 升麻素苷 5-O-甲基维斯阿米醇苷 茅术醇 Β-桉叶醇 苍术素 防风 苍术 

分 类 号：R917[医药卫生—药物分析学]