小檗碱对腹腔暴露后大鼠肠黏膜屏障的影响  

Effect of berberine on intestinal mucosa barrier in rat induced by peritoneal air exposure

作　　者：谈善军[1,2,3] 虞文魁[1,2] 林志亮[1,2] 董翼[1,2] 段开鹏 白小武[4,2] 徐琳[4,2] 李宁 

机构地区：[1]南京大学医学院附属金陵医院 [2]南京军区南京总医院解放军普通外科研究所,江苏南京210002 [3]复旦大学附属中山医院普通外科,上海200032 [4]第二军医大学南京临床学院

出　　处：《肠外与肠内营养》2015年第5期291-294,共4页Parenteral & Enteral Nutrition

基　　金：全军十二五重大新上项目(AWS12J001);江苏省临床医学科技专项基金(BL2012006)

摘　　要：目的:探讨小檗碱对腹腔暴露后大鼠肠黏膜屏障的影响。方法:将大鼠剖腹后暴露腹腔,但不进行任何手术操作,暴露3 h后关腹,制作腹腔暴露模型。将30只大鼠随机分为五组,即对照组、腹腔暴露组、小檗碱低剂量(100 mg/kg)干预组、中剂量(150 mg/kg)干预组和高剂量(200 mg/kg)干预组,每组6只。小檗碱低、中、高剂量干预组分别于术前3 d和术后6 h每天给予一次相应剂量的小檗碱灌胃。对照组和腹腔暴露组在相同条件下分别给予等量的等渗盐水灌胃。术后24 h取血检测血清D-乳酸水平,并取末端回肠组织测定肠道FD4清除率,同时观察末端回肠组织的病理变化,并进行肠黏膜损伤Chiu's评分。结果:与对照组比,腹腔暴露组大鼠血清D-乳酸水平明显上升(P<0.05),肠道FD4清除率和肠黏膜损伤Chiu's评分明显增加(P<0.05)。与腹腔暴露组比,各小檗碱干预组大鼠血清D-乳酸水平有所下降,肠道FD4清除率和肠黏膜损伤Chiu's评分有所降低,以中、高剂量组最为显著(P<0.05)。结论:小檗碱可减轻腹腔暴露引起的肠黏膜损伤,对维持肠黏膜屏障功能具有一定的保护作用。Objective ： To investigate the effect of berberine on intestinal mucosa barrier in rat in- duced by peritoneal air exposure. Methods ： The laparotomy was used, without any surgical procedure, but with peritoneal air exposure for 3 h, to induce the model for peritoneal air exposure. Thirty rats were randomized into five groups：a control group, a peritoneal air exposure model group, and three berberine treatment groups with low （100 mg/kg） , moderate （150 mg/kg） and high dose （200 mg/kg）, respec- tively （ n = 6 each）. At 24 h after surgery, blood was obtained to determinate serum D-lactate, terminal ileum was sampled to determinate intestinal clearance of FD4 and to observe histological changes and Chiug score for damage of intestinal mucosa. Results： Compared with the control group, the model group showed a significant increase in serum D-lactate, intestinal clearance of FD4 and Chiu＇s score for damage of intestinal mucosa （ P 〈 0. 05 ）. Compared with the model group, three berberine treatment groups showed different degrese of decrease in serum D-lactate, intestinal clearance of FD4 and Chiu＇s score. exposure Conclusion： The berberine could decrease intestinal mucosal damage induced by peritoneal air and further maintain intestinal mucosal barrier.

关 键 词：腹腔暴露 小檗碱 肠黏膜屏障 肠通透性 D-乳酸 动物 

分 类 号：R285.5[医药卫生—中药学]