非节段型白癜风患者外周血Th17细胞亚群及其自噬相关标记分子表达  

Expression of Th17 Cells and its Autophagy-related Markers in Peripheral Blood of Patients with Non-segemental Vitiligo

作　　者：朱英杰[1] 刘仲荣[1] 严苗苗[1] 陈胡林[1] 赵敏玲[1] 李翠华[1] 

机构地区：[1]广州军区广州总医院皮肤科,广东广州510010

出　　处：《中国皮肤性病学杂志》2015年第10期1012-1016,共5页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(30972652);广东省自然科学基金资助项目(915100100200020)

摘　　要：目的探讨非节段型白癜风患者(non-segmental vitiligo,NSV)外周血Th17细胞亚群比例及其细胞自噬水平是否存在异常。方法分离人外周血单个核细胞(PBMC),应用流式细胞术检测40例NSV患者及20例正常人外周血Th17细胞亚群比例及其自噬相关标记分子的水平。结果 NSV快速进展期、稳定期至健康对照组外周血Th17细胞亚群比例逐渐降低,各组间差异有统计学意义(P<0.05);Th17细胞自噬相关标记分子LC3B和Beclin1的水平自NSV快速进展组、稳定期组至健康对照组依次降低,而Bcl-2蛋白的水平自NSV快速进展期组、稳定期至健康对照组依次升高,各组间差异均有统计学意义(P均<0.05)。结论 NSV患者外周血Th17细胞亚群计数及其自噬水平均升高,可能参与了NSV病情的发生、发展机制。Objective To detect the expression of Th17 cells and its autophagy-related markers in peripheral blood of patients with non - segmental vitiligo （NSV） . Methods The expression of Thl7 cells and its autophagy-related markers in peripheral blood from 40 NSV patients and 20 healthy controls were detected by flow cytometry. Results The level of Thl7 ceils of NSV patients was significantly higher. In addition, significantly increased expression of Beclinl and LC3B were also observed in NSV patients, whereas the level of BC1-2 was decreased （ all P 〈 0. 05 ）. Conclusion The level of Th17 cells of NSV patients was significantly higher as well as the activity of autophagy. Greater insights into the mechanisms of interaction between Th17 cells and autopgagy are necessary. This research could provide vital new information on the role of Th17 ceils in the pathogenesis of NSV.

关 键 词：非节段型 白癜风 TH17细胞 自噬 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]