香蕉类甜蛋白基因MaTLP1的克隆与表达分析  

Cloning and Expression Analysis of Banana MaTLP1 Gene

作　　者：李健平[1] 王卓[2] 徐碧玉[2] 金志强[2,3] 

机构地区：[1]海南大学,海口571101 [2]中国热带农业科学院热带生物技术研究所,海口571101 [3]中国热带农业科学院海口实验站,海口570102

出　　处：《西北植物学报》2015年第9期1746-1751,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金(ITBB2015ZY15);国家现代农业产业体系项目(CARS-32)

摘　　要：在香蕉EST文库中,通过RACE技术克隆到1个香蕉类甜蛋白基因的全长序列。该序列最大开放阅读框942bp,编码313个氨基酸。Blast分析发现,它与其他类甜蛋白相似度为56.10%,含有类甜蛋白(TLPs)特有的保守结构域,命名为MaTLP1。系统进化树表明,MaTLP1基因编码蛋白与海枣的亲缘关系较近,与香蕉的进化模式相似。组织特异性分析表明,MaTLP1在根、球茎、假茎中的表达量高,叶中较弱,花和果实中微量表达。实时荧光定量PCR分析显示,在抗病香蕉品种中,接种尖孢镰刀菌古巴专化型(Fusarium oxysporum f.sp.cubense,Foc)枯萎病菌后MaTLP1基因上调表达,在感病香蕉品种接菌2d后MaTLP1基因受到抑制,虽然在接菌4d后上调表达,但是相对于抗病品种上调较小。研究表明,MaTLP1基因可能在香蕉抗枯萎病的过程中起作用。Based on banana EST library,we cloned a cDNA of TLP protein from Musa acuminata subsp.malaccensis.This cDNA has an open reading frame of 942 nucleotides,which encodes a protein of 313 amino acids.Blast found that its sequence has a high identity with other thaumatin-like proteins（TLPs）and a specific conserved domain TLPs share was found by using Conserved Domain Search Service（CD Search）tool in NCBI and then we named it MaTLP1.Phylogenetic tree indicated that this protein has a close genetic relationship with Phoenix dactylifera.Moreover,it has similar evolutional pattern with M.acuminatasubsp.malaccensis.Organ specific expression analysis showed that expression of MaTLP1 was high in roots,corms,cauloids and weak in leafs,flowers and fruits.Real-time PCR analysis showed that expression of MaTLP1 was highly increased in resistant banana cultivars against pathogen Fusarium oxysporum f.sp.cubense（Foc）and was fluctuated not obviously in susceptible banana cultivars related to resistant cultivars.These results revealed that MaTLP1 plays an important role in banana M.acuminate resistance against pathogen Fusarium oxysporumf.sp.cubense.

关 键 词：类甜蛋白 香蕉 克隆 表达 

分 类 号：Q786[生物学—分子生物学] Q789