核桃JrsHSP17.3基因克隆及温度胁迫响应模式分析  

JrsHSP17.3 Cloning from Juglans regia and Its Expression Profiles in Response to Temperature Stresses

作　　者：杨桂燕[1] 贾彩霞[1] 孙宇栋[1] 李鸣[1] 翟梅枝[1] 

机构地区：[1]西北农林科技大学林学院核桃研究中心,陕西杨陵712100

出　　处：《西北植物学报》2015年第9期1752-1756,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：西北农林科技大学博士科研启动基金(2014BSJJ038);陕西省科技统筹项目(2012KTZB02-01-01)

摘　　要：通过分析核桃（Juglans regia）转录组,鉴定获得核桃热激蛋白家族sHSP17.3基因的全长cDNA序列,命名为JrsHSP17.3。JrsHSP17.3基因开放读码框474bp,编码产物含157个氨基酸,分子量为17.64kD,理论等电点为5.35。为了研究该基因表达的组织特异性及温度响应模式,采用实时荧光定量PCR（qRT-PCR）方法,对高温和低温胁迫下JrsHSP17.3基因在根、茎、叶中的转录水平进行分析。结果显示,JrsHSP17.3基因在不同组织中均有表达;经高温（36℃~52℃）和低温（16℃~6℃）处理后,JrsHSP17.3基因均可被显著诱导,其中12℃胁迫1h表达最高,为对照（非处理情况）的142.02倍。研究表明核桃JrsHSP17.3基因能够响应冷热温度胁迫,为进一步研究JrsHSP17.3基因的抗寒耐高温特性奠定基础。The full length cDNA of a sHSP17.3was identified by analysis of the transcriptome of Juglans regia,which was named as JrsHSP17.3.The ORF of JrsHSP17.3is 474 bp in length and encodes a 17.64 kDa protein with 157 amino acid residues and the theoretical pI of 5.35.To understand the tissue specific and temperature responsive expression profiles,we analyzed the transcription levels of JrsHSP17.3in roots,stems and leaves exposed to heat and cold stresses at different time points by quantitative real time PCR（qRT-PCR）.The results showed that JrsHSP17.3was expressed more or less in stems,roots and leaves,and was obviously induced after high（36 ℃-52 ℃）and low（16 ℃-6 ℃）temperature treatments,among which the highest expression level was 142.02-fold of control under 12℃for 1h,suggesting JrsHSP17.3could response to heat and cold temperature stresses.These results will provide a basis for further understanding the function of JrsHSP17.3in cold and heat tolerance.

关 键 词：核桃 热激蛋白 JrsHSP17.3 组织表达 温度胁迫 

分 类 号：Q786[生物学—分子生物学]