乌拉尔图小麦Viviparous-1基因的单倍型及其表达特性研究(英文)  

Identification and Characterization of Viviparous-1 Haplotypes in Wheat Related Species Triticum urartu

作　　者：刘思蒙[1] 孙晓燕[1] 刘世鑫[1] 曲若端[1] 王晓丽[1] 杨燕[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《西北植物学报》2015年第9期1757-1766,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31260320,31160247);内蒙古自然科学基金(2014MS0338);内蒙古农业大学科技创新团队基金(NDPYTD2010-3)

摘　　要：该研究采用PCR和半定量RT-PCR方法,对乌拉尔图小麦(Triticum urartu)休眠基因Viviparous-1A(Vp-1A)的单倍型进行分析,并通过建立系统进化树,对Vp-1A基因在乌拉尔图小麦、普通小麦和其他近缘种间的系统发育关系进行分析。结果表明:(1)在20份乌拉尔图小麦中共发现4种新等位变异类型,分别命名为TuVp-1Abgi、TuVp-1Adfi、TuVp-1Aefi和TuVp-1Acgh。与普通小麦的TaVp-1Aa(AJ400712)基因相比,这4种单倍型主要是在第3内含子中有多个TTC不同重复,在第2和第5内含子中存在序列的缺失以及SNPs;(2)用ABA处理种子胚后,4种不同单倍型材料的mRNA表达水平发生变化,表明这4种单倍型对ABA敏感性不同;(3)乌拉尔图小麦中Vp-1A基因不同单倍型,在第2、第3和第5内含子中碱基序列的插入和缺失,影响了Vp-1A基因的表达特性及对ABA的敏感性,从而影响种子休眠特性;(4)鉴定和分析发现,乌拉尔图小麦TuVp-1Adfi单倍型可作为小麦穗发芽抗性资源。The object of this study was to identify and characterize the haplotypes of Vp-1Ain Tritivum urartu by PCR and semi-quantitative RT-PCR and their phylogenetic relationship with other wheat related species.In total,4novel Vp-1Ahaplotypes were explored among a collection of 20 T.urartugenotypes and designated as TuVp-1Abgi,TuVp-1Acgh,TuVp-1Adfi and TuVp-1Aefi,respectively.The main differences between TaVp-1Aa（AJ400712）in common wheat and the newly identified haplotypes were the numbers of TTC repeat,the deletions and SNPs in second and fifth introns.In addition,the expression levels of Vp-1Ahomologues were different in genotypes harboring these 4haplotypes.Furthermore,different mRNA levels among the four genotypes were observed after ABA treatment,indicating their different responsiveness to ABA exposure.These results suggested that the indels in the second,third and fifth intron regions of the Vp-1A gene affected its expression and its sensitivity to ABA,and thus seed dormancy.Therefore,our study characterized the Vp-1A haplotypes in a set of T.urartu germplasm and identified a novel haplotype TuVp-1Adfi which might be useful in wheat improvement for PHS resistance.

关 键 词：乌拉尔图小麦 Vp-1A RT-PCR ABA敏感性 

分 类 号：Q75[生物学—分子生物学]