植绒和棉签拭子对不同载体微量生物物证的提取研究  

Trace DNA Extraction from Three Kinds of Carrier Using 4N6FLOQSwabs^(TM) and Nano-cotton Swab

作　　者：徐海军 叶志鹏 徐姚力 张金燕 李丽 

机构地区：[1]海宁市公安司法鉴定中心,浙江海宁314400

出　　处：《刑事技术》2015年第5期373-375,共3页Forensic Science and Technology

摘　　要：目的比较4N6FLOQSwabsTM植绒拭子和复合纳米棉签拭子对微量生物样本DNA的提取效果。方法选取常见的三种载体(玻璃、一字开刀、抽屉拉手),经消毒去污处理,模拟载体表面微量DNA样本,分别采用4N6FLOQSwabsTM植绒拭子和复合纳米棉签拭子提取载体上微量DNA样本,以检出STR分型基因座数量、等位基因丢失数量、峰高面积三个方面作为提取效果的比较指标。结果 4N6FLOQSwabsTM植绒拭子提取微量DNA样本组成功检出5份微量DNA样本,有效检出4份;复合纳米棉签拭子提取微量DNA样本组中9份微量DNA样本均有效检出;复合纳米棉签拭子提取组的等位基因丢失数量显著多于4N6FLOQSwabsTM,峰高面积显著低于4N6FLOQSwabsTM。结论 4N6FLOQSwabsTM植绒拭子对本研究中的玻璃表面指纹印、开刀表面脱落细胞和抽屉拉手表面脱落细胞的DNA提取效果显著优于复合纳米棉签拭子。Objective To compare the effect of the 4N6FLOQSwabsTM and Nano-cotton swab on extracting trace DNA. Methods Three kinds of pre-decontaminated carriers, glass, screw driver and drawer＇s handle, were used to contact volunteers＇ hands and 9 pairs of samples of human trace DNA （fingerprints on glass, exfoliated ceils on screwdrivers and drawer handles） were prepared. DNA was extracted by either 4N6FLOQSwabs^TM and/or Nano-cotton swab from all the samples and analyzed with GeneMapper ID V3.2 software. The DNA extract efficiency was judged by the quantity of the detected loci, the loss of allelic genes and the peak area. Results For the 4N6FLOQSwabs^TM, five samples were successfully detected and the other four were effectively detected. For the Nano-cotton swab, all the nine samples were detected just at the effective level along with more significant loss of allelic gene and more remarkable lower peak area than the corresponding one of the 4N6FLOQSwabs^TM. Conclusions The effect of extracting trace DNA using 4N6FLOQSwabs^TM is better than that using Nano- cotton swab.

关 键 词：法医遗传学 微量DNA DNA提取 4N6FLOQSwabs^TM植绒拭子 复合纳米棉签拭子 

分 类 号：DF795.2[医药卫生—法医学]